Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
1999-06-10
2002-01-01
Nguyen, Bao-Thuy L. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C422S051000, C422S067000, C435S007900, C435S007940, C435S287700, C436S513000, C436S518000, C436S531000, C436S823000
Reexamination Certificate
active
06335171
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The invention is concerned with a method for the detection of substances contained on textile fibers or other natural or synthetic polymers.
2. Description of Related Technology
Textiles or foods, e.g., luxury foods, have numerous substances contained in them which could act as allergens for consumers, depending on immunological disposition. These allergens trigger allergic reactions which are manifested with an extensive symptomatology, mostly by strong release of histamine. Erythema, irritation of mucosa, asthmatic reactions, or even drop in blood pressure with shock-like states may result in allergic persons. Therefore, it is of great importance for allergic persons to identify substances that represent a risk potential according to the particular individual allergic profile in textiles and foods, e.g., luxury foods, etc., with a rapid process that is easy to perform.
U.S. Pat. No. 5,324,642 describes a method for the analysis of an analyte in a keratin structure (e.g., hair, nails), in which an enzyme and a compound with low redox potential are allowed to act on a sample of the keratin structure, in order to degrade the structure and to dissolve the analyte in this digesting solution. The detection of the analyte is preferably done by immunoassay techniques on a protein basis (that is, with antibodies).
In Dewair et al., J. Allergy Clin. Immunol. 76 (4), 537-542 (1985), “Use of immunoblot technique for detection of human IgE and IgG antibodies to individual silk proteins”, silk proteins are extracted from silk, separated with the aid of polyacrylarnide gel electrophoresis (that is, not with antibodies) and incubated with human serum which may contain IgE- and IgG-antibodies against silk proteins. The detection of the antibodies bound to the silk proteins is carried out using the immunoblot technique.
In B. M. Hausen et al., Deutsche Medizinische Wochenschrift, 109 (39), 1469-1475 (1984), “Hosiery Dye Allergy”, it is reported that test persons with hosiery allergy reacted allergically in the epicutaneous test to isolated hosiery dyes (especially azo dyes) separated using chromatography. A detection of the allergens with the antibodies is not described.
SUMMARY OF THE INVENTION
The invention provides a method for detecting or quantitatively determining substances contained in textile fibers and/or other natural or synthetic polymers with the aid of antibodies whereby a consumer can check the allergenic potential of a textile or of a food such as a luxury food before purchasing it.
According to the invention, a substance contained on a textile fiber or polymer substrate is detected by directly contacting a surface of the substrate and the substance contained therein with antibodies that specifically bind to the substance, and detecting or quantitatively determining the antibodies bound to the substance.
REFERENCES:
patent: 5169757 (1992-12-01), Yamazaki et al.
patent: 5324642 (1994-06-01), Baumgartner
patent: 5840587 (1998-11-01), Stewart et al.
Hemmila, I., Clin. Chem., 31(3):359-370, 1985.*
International Preliminary Examination Report dated Mar. 12, 1999 in PCT/EP97/06763.
“Use of Immunoblot Technique for Detection of Human IgE and IgG antibodies to Individual Silk Proteins”, Dewair et al.; J. Allergy Clin. Immunol, Oct. 1985, pp. 537-342.
“Strumpffarben-Allergie”, Hausen et al.; Deutsche Medizinische Wochenschrift, 109, Sep. 1984, pp. 1469-1475 (English abstract only).
“Immunoglobulin E Antibodies Against a Reactive Dye” Hagmar et al.; Scand J. Work Environ Health 12 (1986), pp. 221-222.
“Fluorescence Photometric Detection of Sizing Agents”, Döbel et al.; Melliand Textilberichte May 1996, pp. 298-300 (with translation).
Journal abstract, Fischer et al., 1990.
Brosius Rupert
Hock Bertold
Langhals Heinz
Diagnostic SYstems Biotechnologie GmbH
Marshall O'Toole Gerstein Murray & Borun
Nguyen Bao-Thuy L.
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