Apparatus and method for separating an analyte

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S644000

Reexamination Certificate

active

07390389

ABSTRACT:
The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.

REFERENCES:
patent: 2815320 (1957-12-01), Kollsman
patent: 3719580 (1973-03-01), Roberts et al.
patent: 3888758 (1975-06-01), Saeed
patent: 4148703 (1979-04-01), Trop et al.
patent: 4362612 (1982-12-01), Bier
patent: 4971670 (1990-11-01), Faupel et al.
patent: 5082548 (1992-01-01), Faupel et al.
patent: 5114555 (1992-05-01), Stimpson
patent: 5126022 (1992-06-01), Soane et al.
patent: 5149418 (1992-09-01), Flesher
patent: 5540826 (1996-07-01), Bier et al.
patent: 5773645 (1998-06-01), Hochstrasser
patent: 5834272 (1998-11-01), Righetti
patent: 6007865 (1999-12-01), Cerami et al.
patent: 6013165 (2000-01-01), Wiktorowicz et al.
patent: 6328869 (2001-12-01), Ogle
patent: 6638408 (2003-10-01), Speicher et al.
patent: 6706162 (2004-03-01), Voss et al.
patent: 2003/0104449 (2003-06-01), Faupel et al.
patent: 2051715 (1972-04-01), None
patent: 0173081 (1986-03-01), None
patent: 0457526 (1991-11-01), None
patent: 0776700 (1997-06-01), None
patent: 1422118 (1976-01-01), None
patent: WO 00/74850 (2000-12-01), None
patent: WO 01/68225 (2001-09-01), None
patent: WO 01/75432 (2001-10-01), None
patent: WO 01/86279 (2001-11-01), None
Righetti et al. “Protein purification in multicompartment electrolyzers with isoelectric membranes”, Journal of Chromatography B, 1997, vol. 699, pp. 105-115.
Righetti et al. “Preparative protein purification in a multi-compartment electrolyser with immobiline membranes”, Journal of Chromatography, 1989, vol. 475, pp. 293-309.
Zuo et al. “A method for Global Analysis of Complex Proteomes Using Sample Prefractionation by Solution Isoelectrofocusing Prior to Two-Dimensional Electrophoresis”, Sep. 10, 2000, Analytical Biochemistry vol. 284, pp. 266-278.
Rylatt et al. “Electrophoretic transfer of proteins across polyacrylamide membranes”, 1999, Journal of Chromatography A, vol. 865, pp. 145-153.
Righetti et al. “Preparative electrophoresis with and without immobilized pH gradients”, 1992, Advances in electrophoresis, vol. 5, pp. 159-200.
G. Kemp, “Capillary Electrophoresis: a Versatile Family of Analytical Techniques”, Biotechnol. Appl. Biochem., No. 27, pp. 9-17, Jun. 1998.
Koegler et al., “Focusing Proteins in an Electric Field Gradient”, Journal of Chromatography A, vol. 229, pp. 229-236, Mar. 1996.

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