MN gene and protein

Details MN gene and protein MN gene and protein

2011-03-22

2011-03-22

Rawlings, Stephen L (Department: 1643)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

C530S328000

Reexamination Certificate

active

07910691

ABSTRACT:
Identified herein is the location of the MN protein binding site, and MN proteins/polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins/polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked by organic or inorganic molecules, preferably organic molecules, more preferably proteins/polypeptides that specifically bind to that site. Therapeutic methods for inhibiting the growth of preneoplastic
eoplastic vertebrate cells that abnormally express MN protein are disclosed. Vectors are provided that encode the variable domains of MN-specific antibodies and a flexible linker polypeptide separating those domains. Further vectors are disclosed that encode a cytotoxic protein/polypeptide operatively linked to the MN gene promoter, and which vectors preferably further encode a cytokine. The MN gene promoter is characterized, and the binding site for a repressor of MN transcription is disclosed.

REFERENCES:
patent: 5387676 (1995-02-01), Zavada et al.
patent: 5585479 (1996-12-01), Hoke et al.
patent: 8808854 (1988-11-01), None
patent: 9318152 (1993-09-01), None
patent: 9534650 (1995-12-01), None
Zavada et al. (Br. J. Cancer. Jun. 2000; 82 (11): 1808-1813).
Anton et al., “Localized renal-cell carcinoma: detection of abnormal cells in peritumoral tissue. A cytophotometry and immunocytochemistry study,”World J. Urol., 13(3): 149-152 (1995).
Bander et al., “Renal cancer imaging with monoclonal antibody G250,”J. Urol., 155 (5 Suppl.): 583A (Abstract 1088) (1996).
Brewer et al., “A Study of Biomarkers in Cervical Carcinoma and Clinical Correlation of the Novel Biomarker MN,”Gynecologic Onocology, 63: 337-344 (1996).
Cho et al., “Hypomethylation of MN/CA9 promoter region contributes to its expression in human renal cell carcinomas,” Abstract 2922,Proceedings of the American Association for Cancer Research, Apr. 10-14, 1999, Philadelphia, PA, vol. 40 (Mar. 1999).
Costa et al., “MN Protein Immunolocalization in Uterine Cervix Carcinoma With Glandular Differentiation—A Clinicopathologic Study of a New Cancer-specific Biomarker,”International Journal of Surgical Pathology, 3(2): 73-82 (1995).
Costa, M., “MN and Ki67 (MIB-1) in Uterine Cervix Carcinoma: Novel Biomarkers With Divergent Utility,”Human Pathology, 27(3): 217-219 (Mar. 1996).
Cote, “Protein Antigen Helps Identify Early Cervical Abnormalities,”Women's Health Weekly: News Section, p. 7 (Mar. 30, 1998).
Divgi et al., “Scintigraphy of Renal Cell Carcinoma with I-131 Labelled Monoclonal Antibody (MAB) G250,”European Journal of Nuclear Medicine, 19(8): 578 (Abstract 121-3) (Aug. 23, 1992).
Divgi et al., “Radioimmunotherapy (RIT) with I-131 Monoclonal Antibody (Mab) G250 in Metastatic Renal Cancer,”Proceedings of the 41stAnnual Meeting, 35(5): 101P (Abstract #401) (May 1994).
Divgi et al., “Radioimmunotherapy with I-131-G250 in Metastatic Renal Cell Cancer (RCC),”J. Nucl. Med., 36 (5 Suppl.): 913P (Abstract 956; May 1995).
Frohman et al., “Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer,”PNAS(USA), 85: 8998-9002 (Dec. 1988).
Frosch et al., “Cloning and characterisation of an immunodominant major surface antigen ofEchinococcus multilocularis,” Molecular and Biochemical Parasitology, 48: 121-130 (1991).
Ivanov et al., “Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes,”PNAS(USA) 95: 12956-12601 (Oct. 1998).
Ivanov et al., “Activation of transmembrane carbonic anhydrases in cancer development,” Abstract 4549,Proceedings of the American Association for Cancer Research, Apr. 10-14, 1999, Philadelphia, PA, vol. 40 (Mar. 1999).
Karttunen et al., “Colorectal Tumors Show Abnormal Expression of MN/CA IX,”Pathology Research and Practice 193/5-6: 392 Abstract No. P198 (Abstract from European Congress on Pathology at Maastricht; Aug. 31-Sep. 4, 1997).
Kranenborg et al., “Development and Characterization of Anti-Renal Cell Carcinoma X Antichelate Bispecific Monoclonal Antibodies for Two-Phase Targeting of Renal Cell Carcinoma,”Cancer Res., 55 (23 Suppl.) 5864s-5867s (1995).
Kurth et al., “Characterization of Human Renal Cell Carcinoma Tumor Lines by Means of Monoclonal Antibodies,”Prostate, 6(4): 451 (Abstract) (1985).
Liao et al., “Identification of the MN Antigen as a Diagnostic Biomarker of Cervical Intraepithelial Squamous and Glandular Neoplasia and Cervical Carcinomas,”American Journal of Pathology, 145(3): 598-609 (Sep. 1994).
Liao and Stanbridge, “Expression of the MN Antigen in Cervical Papanicolaou Smears Is an Early Diagnostic Biomarker of Cervical Dysplasia,”Cancer Epidemiology, Biomarkers&Prevention, 5: 549-557 (Jul. 1996).
Liao et al., “Identification of the MN/CA9 Protein As a Reliable Diagnostic Biomarker of Clear Cell Carcinoma of the Kidney,”Cancer Research, 57: 2827-2831 (Jul. 15, 1997).
Liao and Stanbridge, “Expression of MN/CA9 Protein in Papanicolaou Smears Containing Atypical Glandular Cells of Undetermined Significance Is a Diagnostic Biomarker of Cervical Dysplasia and Neoplasia,”Cancer, 88(5): 1108-1121 (2000).
Lieskovska et al., “Study of in vitro conditions modulating expression of MN/CA IX protein in human cell lines derived from cervical carcinoma,”NEOPLASMA, 46: 17-24 (1999).
Luiten et al., “Target-Specific Activation of Mast Cells by Immunoglobulin E Reactive with a Renal Cell Carcinoma-Associated Antigen,”Laboratory Investigation, 74(2): 467-475 (1996).
Luiten et al., “Generation of chimeric bispecific G250/anti-CD3 monoclonal antibody, a tool to combat renal cell carcinoma,”British Journal of Cancer, 74(5): 735-744 (1996).
Luner et al., “Monoclonal Antibodies to Kidney and Tumor-associated Surface Antigens of Human Renal Cell Carcinoma,”Cancer Res., 46(11): 5816-5820 (1986).
McKiernan et al., “Expression of the Tumor-associated GeneMN: A Potential Biomarker for Human Renal Cell Carcinoma,”Cancer Research, 57: 2362-2365 (Jun. 15, 1997).
McKiernan et al., “Molecular Detection of Clear Cell Carcinoma of the Kidney Via a Blood Test for MN/CA9 and Prostate Specific Membrane Antigen,” (Abstract No. 1272) 35thASCO Annual Meeting in Atlanta, GA (May 15-18, 1999).
McKiernan et al., “First kidney cancer blood test may prevent organ removal, say researchers,”Clinica, 859: 18 (May 24, 1999).
McKiernan et al., “The Detection of Renal Carcinoma Cells in the Perpheral Blood with an Enhanced Reverse Transcriptase-Polymerase Chain Reaction Assay for MN/CA9,”Cancer, 86(3): 492-497 (Aug. 1, 1999).
Moon et al., “A Highly Restricted Antigen for Renal Cell Carcinoma Defined by a Monoclonal Antibody,”Hybridoma, 4(2): 163-172 (1985).
Mulders et al., “G250, A Tumor Antigen with Therapeutic Potential in Renal Cell Carcinoma (RCC),”Journal of Urology, 164(4): Abstract 652 (Apr. 1999).
Murakami, et al., “MN/CA9gene expression as a potential biomarker in renal cell carcinoma,”BJU International, 83: 743-747 (1999).
Nakagawa et al., “MN as a Potential Target in Renal Cell Carcinoma,”J. Urology, 159 (5 Suppl.): 187, Abstract 720 (May 1998).
Nakagawa, et al., “MN targeting immunotherapy for human renal cell carcinoma: Curative protective tools,” Abstract 3136,Proceedings of the American Association for Cancer Research, Apr. 10-14, 1999, Philadelphia, PA, vol. 40 (Mar. 1999).
Oosterwijk and Debruyne, “Radiolabeled monoclonal antibody G250 in renal-cell carcinoma,”World Journal of Urology, 13: 186-190 (1995).
Oosterwijk et al., “The Expression of Renal A

Affiliated with

Also associated with

Rating

MN gene and protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with MN gene and protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and MN gene and protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2777155