Method of screening endothelial cells for angiogenic capability

Details Method of screening endothelial cells for angiogenic capability Method of screening endothelial cells for angiogenic capability

2011-08-23

2011-08-23

Huynh, Phuong (Department: 1644)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007210, C435S007500, C435S007920, C530S387100, C530S388220, C530S391300

Reexamination Certificate

active

08003336

ABSTRACT:
Provided is a method of screening a primary endothelial cell population for angiogenesis capability comprising: (a) measuring the percentage of cells that are positive for VEGF R2 and CD34, the level of VEGF R2, or measuring the VEGF R2 to VEGF R1 ratio in the population; and (b) selecting those populations where the measured percentage or the measured ratio is over a threshold value.

REFERENCES:
patent: 6524583 (2003-02-01), Thorpe et al.
patent: 2003/0100107 (2003-05-01), Peschle
BÖldicke et al, Stem cells 19: 24-36, 2001.
Kanda et al, Endothelium 6(1): 33-44, 1998.
Asahara et al., Science 275: 964-967, Feb. 1997.
Favier et al., Angiogenesis and Vascular Architecture in Pheochromocytomas, American Journal of Pathology, Oct. 2002, pp. 1235-1246, vol. 161, No. 4.
Favre, Expression of genes involved in vascular development and angiogenesis in endothelial cells of adult lung, AJP-Heart and Circulatory Physiology, Nov. 2003, pp. 1917-1938, vol. 285.
Folkman, Tumor Angiogenesis: Therapeutic Implications, New England Journal of Medicine, Nov. 18, 1971, p. 1182-1186, vol. 285, No. 21.
Harlow et al., in Antibodies: a laboratory manual, 1988, Cold Spring Harbor Laboratory publication, Cold Spring Harbor, NY, pp. 321-323 and 409-410.
Khan et al., Clinical Cytometry 64B: 1-8, 2005.
Peichev et al., Blood 95(3): 952-958, Feb. 2000.
Richard et al., Experimental Cell Research 240: 1-6, 1998.
Sato, Correlations of the Expression of Fibroblast Growth Factor-2, Vascular Endothelial Growth Factor, and their Receptors with Angiogenesis in Synovial Tissues from Patients with Internal Derangement . . . , J Dent Res, 2003, pp. 272-277, vol. 82(4).
Takeshita, Intramuscular Administration of Vascular Endothelial Growth Factor Induces Dose-Dependent Collateral Artery Augmentation in a Rabbit Model of Chronic Limb Ischemia, Circulation, 1994, pp. 11-228, vol. 90, No. 5//PT2.
Yang et al., Arterioscler Thromb Vasc Biol.; 21:1934-1940, 2001.

Affiliated with

Also associated with

Rating

Method of screening endothelial cells for angiogenic capability does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of screening endothelial cells for angiogenic capability, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of screening endothelial cells for angiogenic capability will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2764820