Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2011-08-09
2011-08-09
Rao, Manjunath (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S007100, C435S320100, C435S252300
Reexamination Certificate
active
07993872
ABSTRACT:
This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsyn-thetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
REFERENCES:
patent: 6071892 (2000-06-01), Lawlor et al.
patent: 6927042 (2005-08-01), Schultz et al.
patent: 7045337 (2006-05-01), Schultz et al.
patent: 7083970 (2006-08-01), Schultz et al.
patent: 7183082 (2007-02-01), Schultz et al.
patent: 7199222 (2007-04-01), Shultz et al.
patent: 7217809 (2007-05-01), Schultz et al.
patent: 7238510 (2007-07-01), Schultz et al.
patent: 7262040 (2007-08-01), Schultz et al.
patent: 2004/0198637 (2004-10-01), Schultz et al.
patent: 2004/0265952 (2004-12-01), Deiters et al.
patent: 2005/0009049 (2005-01-01), Chin et al.
patent: 2005/0136513 (2005-06-01), Zhang et al.
patent: 2005/0208536 (2005-09-01), Schultz et al.
patent: 2005/0227318 (2005-10-01), Alfonta et al.
patent: 2006/0063244 (2006-03-01), Schultz et al.
patent: 2006/0068478 (2006-03-01), Schultz et al.
patent: 2006/0160175 (2006-07-01), Anderson et al.
patent: 2006/0177900 (2006-08-01), Anderson et al.
patent: 2006/0234367 (2006-10-01), Schultz et al.
patent: 2007/0009990 (2007-01-01), Alfonta et al.
patent: 2007/0020634 (2007-01-01), Anderson et al.
patent: 2007/0042461 (2007-02-01), Anderson et al.
patent: 2007/0111193 (2007-05-01), Zhang et al.
patent: 2007/0154952 (2007-07-01), Chin et al.
patent: 2007/0166791 (2007-07-01), Chin et al.
patent: 2007/0172915 (2007-07-01), Schultz et al.
patent: 2007/0184517 (2007-08-01), Schultz et al.
patent: 2008/0227153 (2008-09-01), Schultz et al.
patent: 1 424 395 (2004-06-01), None
patent: WO 02/085923 (2002-10-01), None
patent: WO 02/086075 (2002-10-01), None
patent: WO 03/014354 (2003-02-01), None
Q. Wang, T.R. Chan, R. Hilgraf, V.V. Fokin, K.B. Sharpless and M.G. Finn, Bioconjugation by copper(I)-catalyzed azide-alkyne [3+2] cycloaddition. J. Am. Chem. Soc. 125 (2003), pp. 3192-3193.
Edwards et al. A Bacterial Amber Suppressor inSaccharomyces cerevisiaeIs selectively Recognized by a Bacterial Aminoacyl-tRNA Synthetase. Molecular and cell Biology. Apr. 1990, pp. 1633-1641).
Sakamoto et al.Site specific Incorporation of an unnatural amino acid into proteins in mammalian cells Nucleic Acid Research, 2002, vol. 30 No. 21.
Dougerty et al. Unnatural amino acids as probes of protein structure and function. Current opinions in Chemical Biology 2000, 4:645-652).
Barker, et al The tyrosyl-tRNA synthetase fromEscherichia coli: complete nucleotide sequence of the structural gene. FEBS Lett. 150, 419-423, 1982.
Anderson et al., (2002) Exploring the Limits of Codon and Anticodon Size,Chemistry and Biology, 9:237-244.
Blake (2001) Cellular screening assays using fluorescence microscopyCurr. Opin. Pharmacol., 1:533-539.
Bolletta, F. et al., (1996) Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury,Organometallics15:2415-17.
Carboni, B et al., (1993) Aliphatic Amino Azides as Key Building Blocks for Efficient Polyamine Syntheses,J. Org. Chem. 58:3736-3741.
Chin et al., (2002), Addition of p-Azido-L-phenylalanine to the Genetic code ofEscherichia coli, J. Am. Chem. Soc. 124:9026-9027.
Chin and Schultz, (2002), In vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis,Chem BioChem11:1135-1137.
Chin, et al., (2002), Addition of a Photocrosslinker to the Genetic Code ofEscherichia coli, Proc. Natl. Acad.Sci. U. S. A. 99:11020-11024.
Chin, et al., (2003) Progress toward an expanded eukaryotic genetic code,Chem. Biol., 10(6):511-519.
Chin, et al., (2003) An expanded eukaryotic genetic code,Science, 301(5635):964-7.
Cornish, et al., (1996) Site-Specific Protein Modification Using a Ketone Handle,Journal of the American Chemical Society118:8150-8151.
Crisp, G. T.; & Gore, J. (1997) Preparation of Biological Labels with Acetylenic Linker Arms,Tetrahedron53:1505-1522.
Feng et al., (2003), Expanding tRNA recognition of a tRNA synthetase by a single amino acid change,PNAS100(10): 5676-5681.
Forster et al., (2003) Programming peptidomimetic synthetases by translating genetic codes designed de novoPNAS100(11):6353-6357.
Francklyn et al., (2002), Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation;RNA, 8:1363-1372.
Griffin, et al., (1998) Specific covalent labeling of recombinant protein molecules inside live cells,Science281:269-272.
Hamano-Takaku et al., (2000) A mutantEscherichia coliTyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine,Journal of Biological Chemistry, 275(51):40324-40328.
Kiga et al. (2002), An engineeredEscherichia colityrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system,PNAS99(15): 9715-9723, pp. 21-23 missing.
Kiick et al., (2002) Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligtation,PNAS99:19-24.
Lemineux, & Bertozzi, (1996) Chemoselective ligation reactions with proteins, oligosaccharides and cells,TIBTECH, 16:506-513.
Liu, D.R. & Schultz, P.G. (1999) Progress toward the evolution of an organism with an expanded genetic code.PNAS United States96:4780-4785.
Magliery, (2001) Expanding the Genetic Code: Selection of Efficient Suppressors of Four-base Codons and Identification of “Shifty” Four-base Codons with a Library Approach inEscherichia coli, J. Mol. Biol. 307: 755-769.
Mahal, et al., (1997) Engineering chemical reactivity on cell surfaces through oligosaccharide biosynthesis,Science, 276:1125-1128.
Padwa, A. (1991) Intermolecular 1,3-Dipolar Cycloaddtions inComprehensive Organic Synthesis, vol. 4, Ed. Trost, B. M., Pergamon, Oxford, p. 1069-1109.
M. Pasternak, et al., (2000), A new orthogonal suppressor tRNA/aminoacyl-tRNA synthetase pair for evolving an organism with an expanded genetic code,Helvetica Chemica Acta83:2277.
Rostovtsev, et al., (2002) A stepwise Huisgen cycloaddition process: copper(I)-catalyzed regioselective “ligation” of azides and terminal alkynes,Angew. Chem. Int. Ed. 41:2596-2599.
Speers, et al., (2003) Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3+2] cycloaddition,J. Am. Chem. Soc., 125:4686-4687.
Tornøe, et al., (2002) Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides,Org. Chem67:3057-3064.
Wang, et al., (2001), Expanding the genetic code ofEscherichia coli, Science292:498-500.
Wang, et al., (2002), Adding L-3-(2-naphthyl)alanine to the genetic code ofE-coli, J. Am. Chem. Soc. 124:1836-1837.
Wang and Schultz, (2002), Expanding the Genetic Code,Chem. Comm. 1-10.
Wang, et al., (2003) Addition of the keto functional group to the genetic code ofEscherichia coli, Proc. Natl. Acad. Sci., 100:56-61.
Wang, et al., (2003) Bioconjugation by copper(I)-catalyzed azide-alkyne [3+2] cycloaddition.J. Am. Chem. Soc., 125:3192-3193.
Wouters, et al., (2001) Imaging biochemistry inside cells,Trends in Cell Biology11:203-211.
Zacharias, et al., (2000) Recent advances in technology for measuring and manipulating cell signals,Curr. Opin. Neurobiol., 10:416-421.
Zhang et al., (2002), The selective incorporation of alkenes into proteins inEscherichia coli, Angewandte Chemie. International Ed. in English41:2840-2842.
Zhang, et al., (2003) A new strategy for the site-specific modification of proteins in vivo,Biochemistry, 42:6735-6746.
Deiters and Schultz (2005) “In vivo incorporation of an alkyne into proteins inEscherichia coli.” Bioorganic&Medicinal Chemistry Letters, 15: 1521-1524.
Deiters et al. (2003) “Adding Amino Acids with Novel Reactivity to the Genetic Code ofSaccharomyces.” Journal of the American Shemistry Society, 125: 1178
Anderson J. Christopher
Chin Jason W.
Cropp T. Ashton
Deiters Alexander
Schultz Peter G.
Gebreyesus Kagnew H
Landry Stacy
Quine Intellectual Property Law Group
Rao Manjunath
The Scripps Research Institute
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