Method for the simultaneous amplification of multiple...

Details Method for the simultaneous amplification of multiple... Method for the simultaneous amplification of multiple...

2011-01-11

2011-01-11

Chunduru, Suryaprabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S024330

Reexamination Certificate

active

07867701

ABSTRACT:
A method is described for the amplification of nucleic acids, in which the segments to be amplified are first hybridized with at least two primer oligonucleotides that have two domains, wherein the sequence-specific domain found at the 3-end hybridizes to the segment to be amplified, while the generic domain found at the 5-end does not hybridize. Then an amplification reaction is conducted by means of a polymerase and subsequently a labeled primer oligonucleotide, which binds to the generic domain of the first primer, is hybridized to the amplificate which is formed. In the last step, the sequence of the amplificate is investigated.

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Schuelke, “An economic method for the fluorescent labeling of PCR fragments,” Nature Biotechnology, 18:233-4 (Feb. 2000).

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