Inhibition of viral gene expression using small interfering RNA

Details Inhibition of viral gene expression using small interfering RNA Inhibition of viral gene expression using small interfering RNA

2011-03-08

2011-03-08

Whiteman, Brian (Department: 1635)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

Reexamination Certificate

active

07902351

ABSTRACT:
The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.

REFERENCES:
patent: 6001990 (1999-12-01), Wands et al.
patent: 6174868 (2001-01-01), Anderson et al.
patent: 2003/0143732 (2003-07-01), Fosnaugh et al.
patent: 2003/0175772 (2003-09-01), Wang
patent: 2004/0209831 (2004-10-01), McSwiggen et al.
patent: 2004/0259247 (2004-12-01), Tuschl et al.
patent: 2005/0164210 (2005-07-01), Mittal et al.
patent: 2005/0186586 (2005-08-01), Zamore et al.
patent: 2009/0004739 (2009-01-01), Demura et al.
patent: 2010/0112686 (2010-05-01), Ge et al.
patent: 2004-305140 (2004-11-01), None
patent: WO-03/070750 (2003-08-01), None
patent: 2004029281 (2004-04-01), None
patent: WO 2005028646 (2005-03-01), None
patent: WO-2006/031901 (2006-03-01), None
Vickers et al. (2003) J. Biol. Chem. 278:7108-7118.
Kalota et al. (2007) “Design of antisense oligonucleotides and short interfering RNA duplexes (siRNA) targeted to BCL6 rnRNA: Towards rational drug development for specific lymphoma subsets” Blood Cells, Molecules, and Diseases 38:199-203.
Paddison et al. (2002) Genes & Development 16:948-958.
Latham et al., in RNA Interference Technology From Basic Science to Drug Development (Copyright 2005, Ed. Appasani, K), Cambridge University Press, Chapter 10, pp. 153-154.
Birmingham et al., “3′ UTR Seed Matches, but Not Overall Identity, are Associated with RNAi Off-Targets,” Nature Methods, 3(3):199-204 (2006); Addendum: Nature Methods, 3(6):487 (2006).
Blight et al., “Efficient Initiation of HCV RNA Replication in Cell Culture,” Science, 290:1972-1974 (2000).
Bridge et al., “Induction of an Interferon Response by RNAi Vectors in Mammalian Cells,” Nature Genetics, 34(3):263-264 (2003).
Brown et al., “Secondary Structure of the 5′ Nontranslated Regions of Hepatitis C Virus and Pestivirus Genomic RNAs,” Nucleic Acids Research, 20(19):5041-5045 (1992).
Bukh et al., “Sequence Analysis of the 5′ Noncoding Region of Hepatitis C Virus,” Proc. Nat. Acad. Sci. USA, 89:4942-4946 (1992).
Choo et al., “Genetic Organization and Diversity of the Hepatitis C Virus,” Proc. Natl. Acad. Sci. USA, 88:2451-2455 (1991).
Fish et al., “Short-Term Cytotoxic Effects and Long-Term Instability of RNAi Delivered Using Lentiviral Vectors,” BMC Molecular Biology, 5:9 (2004).
Grimm et al., “Fatality in Mice Due to Oversaturation of Cellular MicroRNA/Short Hairpin RNA Pathways,” Nature, 441:537-541 (2006).
Han et al., “Characterization of the Terminal Regions of the Hepatitis C Viral RNA: Identification of Conserved Sequences in the 5′ Untranslated Region and Poly(A) Tails at the 3′ End,” Proc. Natl. Acad. Sci. USA, 88:1711-1715 (1991).
Hannon et al.. “Unlocking the Potential of the Human Genome with RNA Interference,” Nature, 431:371-378 (2004).
Hugle et al., “Current Therapy and New Molecular Approaches to Antiviral Treatment and Prevention of Hepatitis C,” Rev. Med. Virol., 13:361-371 (2003).
Jubin et, al., “Hepatits C Virus Internal Ribosome Entry Site (IRES) Stem Loop IIId Contains a Phylogenetically Conserved GGG Triplet Essential for Translation of IRES Folding,” Journal of Virology, 74(22):10430-10437 (2000).
Kapadia et al., “Interference of Hepatitis C Virus RNA Replication by Short Interfering RNAs,” Proc. Natl. Acad. Sci. USA, 100(4):2014-2018 (2003).
Kawasaki et al., “Short Hairpin Type of dsRNAs that are Controlled by tRNAValPromoter Significantly Induce RNAi-mediated Gene Silencing in the Cytoplasm of Human Cells,” Nucleic Acids Research, 31(2):700-707 (2003).
Kim et al., “Interferon Induction by siRNAs and ssRNAs Synthesized by Phage Polymerase,” Nature Biotechnology, 22(3):321-325 (2004).
Kronke et al., “Alternative Approaches for Efficient Inhibition of Hepatitis C Virus RNA Replication by Small Interfering RNAs,” Journal of Virology, 78(7):3436-3446 (2004).
Lagos-Quintana et al., “Identification of Novel Genes Coding for Small Expressed RNAs,” Science, 294:853-858 (2001).
Layzer et al., “In Vivo Activity of Nuclease-Resistant siRNAs,” RNA, 10:776-771 (2004).
Lieberman et al., “Interfering with Disease: Opportunities and Roadblocks to Harnessing RNA Interference,” TRENDS in Molecular Medicine, 9(9):397-403 (2003).
Marques et al., “A Structural Basis for Discriminating Between Self and Nonself Double-Stranded RNAs in Mammalian Cells,” Nature Biotechnology, 24(5):559-565 (2006).
McCaffrey et al., “Determinants of Hepatitis C Translational Initiation In Vitro, in Cultured Cells and Mice,” Molecular Therapy, 5(6):676-684 (2002).
McCaffrey et al., “RNA Interference in Adult Mice,” Nature, 418:38-39 (2002).
McCaffrey et al., “A Potent and Specific Morpholino Antisense Inhibitor of Hepatitis C Translation in Mice,” Hepatology, 38(2):503-508 (2003).
McHutchison et al., “Future Therapy of Hepatitis C,” Hepatology, 36(5-S1):S245-S252 (2002).
Mercer et al., “Hepatitis C Virus Replication in Mice with Chimeric Human Livers,” Nature Medicine, 7(8):927-933 (2001).
Okamoto et al., “Nucleotide Sequence of the Genomic RNA of Hepatitis C Virus Isolated from a Human Carrier: Comparison with Reported Isolates for Conserved and Divergent Regions,” J. Gen. Virol., 72(PT. 11):2697-2704 (1991) (Abstract).
Pietschmann et al., “Tissue Culture and Animal Models for Hepatitis C Virus,” Clinics in Liver Disease, 7:23-43 (2003).
Qin et al., “Inhibiting HIV-1 Infection in Human T Cells by Lentiviral-Mediated Delivery of Small Interfering RNA Against CCR5,” Proc. Natl. Acad. Sci. USA, 100(1):183-188 (2003).
Radhakrishnan et al., “RNA Interferences as a New Strategy Against Viral Hepatitis,” Virology, 323:173-181 (2004).
Randall et al., “Interfering with Hepatitis C Virus RNA Replication,” Virus Research, 102:19-25 (2004).
Randall et al., “Clearance of Replicating Hepatitis C Virus Replicon RNAs in Cell Culture by Small Interfering RNAs,” Proc. Natl. Acad. Sci. USA, 100(1):235-240 (2003).
Rice, “Fresh Assault on Hepatitis C,” Nature, 426:129-131 (2003).
Robbins et al., “Stable Expression of shRNAs in Human CD34+Progenitor Cells can Avoid Induction of Interferon Responses to siRNAs In Vitro,” Nature Biotechnology, 24(5):566-571 (2006).
Sen et al., “Inhibition of Hepatitis C Virus Protein Expression by RNA Interference,” Virus Research, 96:27-35 (2003).
Seo et al., “Letter to the Editor: Small Interfering RNA-Mediated Inhibition of Hepatitis C Virus Replication in the Human Hepatoma Cell Line Huh-7,” Journal of Virology, 77(1):810-812 (2003).
Seyhan et al., “Complete, Gene-Specific siRNA Libraries: Production and Expression in Mammalian Cells,” RNA, 11(5):837-846 (2005).
Sookoian “New Therapies on the Horizon for Hepatitis C,” Annals of Hepatology, 2(4):164-170 (2003).
Wang et al., “Small Hairpin RNAs Efficiently Inhibit Hepatitis C IRES-Mediated Gene Expression in Human Tissue Culture Cells and a Mouse Model,” Molecular Therapy, 12(3):562-568 (2005).
Wilson et al., “RNA Interference Blocks Gene Expression and RNA Synthesis from Hepatitis C Replicons Propagated in Human Liver Cells,”,Proc. Natl. Acad. Sci. USA, 100(5):2783-2788 (2003).
Yokota et al., “Inhibition of Intracellular Hepatitis C Virus Replication by Synthetic and Vector-Derived Small Interfering RNAs,” EMBO Reports, 4(6):602-608 (2003).
Zhang et al., “Down-Regulation of

Affiliated with

Also associated with

Rating

Inhibition of viral gene expression using small interfering RNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Inhibition of viral gene expression using small interfering RNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inhibition of viral gene expression using small interfering RNA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2739646