Method of biosynthesizing tetrodotoxin

Details Method of biosynthesizing tetrodotoxin Method of biosynthesizing tetrodotoxin

2011-07-12

2011-07-12

Ariani, Kade (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing heterocyclic carbon compound having only o, n, s,...

Reexamination Certificate

active

07977074

ABSTRACT:
The present invention relates methods for the biosynthesis of tetrodotoxin (TTX) involving the steps of obtaining a culture possessing one or more of aVibriospecies, such as through a seed culture, inoculating the culture and tissue extract from a textrodotoxin-bearing organism in a fermenter medium, and isolating and purifying tetrodotoxin from said fermenter, resulting in a yield of TTX of about 0.5 g TTX/L in about 3 to 5 days, such TTX being at least 90% pure.

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Yu et al./ Two novel species of tetrodotoxin-producing bateria isolated from toxic marine puffer fished/ Toxicon44 (2004) 641-647.
Okada et al./ Vibrios Commonly Possess Two Chromosomes/Journal of Bacteriology/ Jan. 2005/ p. 752-757.
Audris Huang/ Synthetic Approaches to Tetrodotoxin 2007.

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