Reagent for measuring clotting time and method for...

Details Reagent for measuring clotting time and method for... Reagent for measuring clotting time and method for...

2011-03-01

2011-03-01

Robinson, Hope A (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

C435S013000

Reexamination Certificate

active

07897333

ABSTRACT:
A reagent for measuring clotting time is described. The reagent comprises a complex of a phospholipid and a recombinant tissue factor obtained by using an insect or a cultured insect cell as a host; and a soluble component derived from the insect or the cultured insect cell.A method for manufacturing the reagent is also described.

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Takayenoki et al. (Biochemical and Biophysical Research Communications, vol. 181, 1991, pp. 1145-1150).
Yoko Takayenoki et al., cDNA and Amino Acid Sequences of Bovine Tissue Factor, Biochemical and Biophysical Research Communications, Dec. 31, 1991, pp. 1145-1150, vol. 181, No. 3, Academic Press, Inc.
Cheryl L. Brucato et al., Expression of recombinant rabbit tissue factor inPichia pastoris, and its application in a prothrombin time reagent, Protein Expression & Purification, 2002, pp. 386-393, vol. 26, Elsevier Science.
Kost, Thomas A. et al, “Baculovirus as versatile vectors for protein expression in insect and mammalian cells”, Nature Biotechnology vol. 23, No. 5, May 2005, pp. 567-575.

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