FRET probes for fluorescent detection of the ESPS gene

Details FRET probes for fluorescent detection of the ESPS gene FRET probes for fluorescent detection of the ESPS gene

2011-07-19

2011-07-19

Chunduru, Prabha (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120, C435S091200, C536S024330

Reexamination Certificate

active

07982026

ABSTRACT:
The present invention relates to a primer pair for the detection of a specific gene, and more particularly to a probe pair for the detection and analysis of an EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene, in which primers complementary to the base sequences in the EPSPS gene are labeled with fluorophores acting as an energy donor and an energy acceptor, respectively. By introducing the inventive probe pair into cells, hybridizing the primers of the probe pair with the target gene and then analyzing the biological cells in a single cell unit using laser fluorescence measurement technique, cells containing the EPSPS gene of GMOs can be detected and the percentage of the GM cells in a cell mixture can also be quantified.

REFERENCES:
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patent: 2004-0012260 (2004-02-01), None
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S. Sixou, et al., ‘Intracellular oligonucleotide hybridization detected by fluorescence resonance energy transfer (FRET)’ In: Nucleic Acids Research, 1994, vol. 22 (4), pp. 662-668.
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