Recombinant attenuated clostridium organisms and vaccine

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...

Reexamination Certificate

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C424S185100, C424S239100, C424S247100, C530S350000

Reexamination Certificate

active

07972604

ABSTRACT:
The present invention discloses attenuatedClostridium perfringensorganisms that express a substantially nontoxic alpha-toxin. The expressed alpha-toxin is a deletion mutein that relative to the alpha-toxin of the mature alpha-toxin ofClostridium perfringensstrain 13, is missing at least nine consecutive amino acid residues including His68. The present invention also discloses attenuated organisms that encode the muteins, as well as the use of such attenuated organisms as vaccines.

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