Method for the quantitative determination of antigens and antibo

Details Method for the quantitative determination of antigens and antibo Method for the quantitative determination of antigens and antibo

1987-11-24

1992-03-03

Rosen, Sam

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 71, 435808, 436513, 436528, 436531, 436532, 436533, 436534, 356320, 356407, G01N 33543, G01N 33546

Patent

active

050932713

ABSTRACT:
A method for the quantitative determination of antigen (or antibody) which comprises adding a sample containing an antigen (or antibody) to a dispersion of an insoluble carrier of fine particle size with an antibody (or antigen) fixed thereto to effect an antigen-antibody reaction, measuring absorbance of the reaction mixture, A.lambda..sub.1 and A.lambda..sub.2, at two different wavelengths, .lambda..sub.1 and .lambda..sub.2, and calculating the concentration of said antigen (or antibody) in the sample from the absorbance ratio A.lambda..sub.1 /A.lambda..sub.2. This method is suitable for the measurment of the amount of an antigen or antibody in samples taken from living bodies using a simple procedure which has high sensitivity.

REFERENCES:
patent: 3813168 (1974-05-01), Honkawa
patent: 4136959 (1979-01-01), Honkawa et al.
patent: 4180327 (1979-12-01), Maeda et al.
patent: 4225233 (1980-09-01), Ogan
patent: 4954435 (1990-09-01), Krauth

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