Aqueous two-phase protein extraction

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435190, 435814, 435816, 210511, 210634, 210767, 422256, 530350, 530412, 530421, 530422, 530423, C07K 312

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active

050932543

ABSTRACT:
An aqueous two-phase protein partitioning system is disclosed which employs polyvinylpyrrolidone as the upper phase and maltodextrin as the lower phase and provides a low-cost system for protein partitioning. The system can also be employed with the amion derivatives of chlorotriazine dyes, which bind in a noncovalent manner with the PVP and serve as a ligand for the proteins to be separated.

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