Chemiluminescent assay for the determination of density of activ

Details Chemiluminescent assay for the determination of density of activ Chemiluminescent assay for the determination of density of activ

1989-09-14

1992-03-03

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 25, 435 34, 436172, C12Q 102

Patent

active

050932381

ABSTRACT:
A chemiluminescent assay for the determination of density or activity of viable cells which comprises incubating viable cells in the presence of a quinone whose reduced form redcues dissolved oxygen resulting in formation of hydrogen peroxide, reacting the hydrogen peroxide with a chemiluminescence reagent in the presence of a fluorescent substance to cause fluorescence and determining density or activity of the cells on the basis of the intensity of fluorescence.

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patent: 4647532 (1987-03-01), Watanabe et al.
patent: 4655969 (1987-04-01), Richter et al.
patent: 4746607 (1988-05-01), Mura et al.
patent: 4959182 (1990-09-01), Schaap
Mosmann, T., Journal of Immunological Methods 65:55-63 (1983), "Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays".
DeLuca, M. and McElroy, W. D., Methods in Enzymology, vol. 57, pp. 3-15 (1978), "Purification and Properties of Firefly Luciferase".
Hastings-Methods in Enzymology, vol. 57 (1978) pp. 125-135.
Yamashoji et al.-Analytical Biochemistry, vol. 181 (1989) pp. 001 to 004.

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