Nucleic acid probes

Details Nucleic acid probes Nucleic acid probes

1986-12-23

1992-03-03

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 75, 435188, 536 23, 536 26, 536 27, 536 28, 536 29, 935 77, 935 78, C12Q 168

Patent

active

050932322

ABSTRACT:
Modified nucleotides are provided which have the structure ##STR1## wherein R.sup.1 is a reactive group derivatizable with a detectable label, R.sup.2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof, R.sup.3 is hydrogen, methyl, bromine, fluorine or iodine, R.sup.4 is hydrogen, an acid-sensitive, base-stable blocking group of an acyl capping group, R.sup.5 is hydrogen or a phosphorus derivative, R.sup.6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.

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