Method of culturing embryonic stem cells with the use of...

Details Method of culturing embryonic stem cells with the use of... Method of culturing embryonic stem cells with the use of...

2011-04-12

2011-04-12

Ton, Thaian N (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S366000, C435S368000

Reexamination Certificate

active

07923246

ABSTRACT:
The present invention provides a clinically applicable method of inducing differentiation of the embryonic stem cells. Specifically, the present invention provides a method of culturing embryonic stem cells, which comprises culturing embryonic stem cells in the presence of an amnion-derived factor, a cell culture obtained by the culture method, and a culture agent/culture kit of embryonic stem cells comprising an amnion-derived factor. The present invention also provides a method of screening an amnion-derived factor having an activity useful for culturing embryonic stem cells with the activity as an index, and an amniocyte-derived factor obtained by the screening method. Furthermore, the present invention provides a method of preparing amniocytes useful for culturing embryonic stem cells, amniocytes obtained by the preparation method, and the like.

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