Amplification vectors based on trans-splicing

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C536S023720, C800S278000

Reexamination Certificate

active

08003381

ABSTRACT:
This invention provides a process of amplification and/or expression of a sequence of interest in a cell by providing for generating within the cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby the amplicon is capable of amplifying in the cell and capable of expressing a sequence of interest.

REFERENCES:
patent: 5869254 (1999-02-01), Sullenger et al.
patent: 6071730 (2000-06-01), Haseloff et al.
patent: 6632980 (2003-10-01), Yadav et al.
patent: WO 92/13090 (1992-08-01), None
patent: WO 00/17342 (2000-03-01), None
patent: WO 02/29068 (2002-04-01), None
Thomas et al. 2001, The Plant Journal 25(4):417-425.

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