Methods and vectors for site-specific recombination

Details Methods and vectors for site-specific recombination Methods and vectors for site-specific recombination

1995-09-01

1998-09-01

Ketter, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 536 231, 536 232, C12N 1563, C07H 2104

Patent

active

058010300

ABSTRACT:
The present invention provides methods for site-specific recombination in a cell, as well as vectors which can be employed in such methods. The methods and vectors of the present invention can be used to obtain persistent gene expression in a cell and to modulate gene expression.
One preferred method according to the invention comprises contacting a cell with a vector comprising an origin of replication functional in mammalian cells located between first and second recombining sites located in parallel. Another preferred method comprises, in part, contacting a cell with a vector comprising first and second recombining sites in antiparallel orientations such that the vector is internalized by the cell. In both methods, the cell is further provided with a site-specific recombinase that effects recombination between the first and second recombining sites of the vector.

REFERENCES:
patent: 4562155 (1985-12-01), Ricciardi et al.
patent: 4797368 (1989-01-01), Carter et al.
patent: 4959317 (1990-09-01), Sauer
patent: 5173414 (1992-12-01), Lebkowski et al.
Anton et al., J. Virol., 69, 4600-4606 (1995).
Argos et al., EMBO J., 5, 433-440 (1986).
Barinaga, Science, 265, 26, 28 (1994).
Baubonis et al., Nucleic Acids Research, 21, 2025-2029 (1993).
Brunelli et al., Yeast, 9, 1309-1318 (1993).
Clontech (Palo Alto, CA) 1995/1996 Catalog, 187-188.
Fiering et al., Proc. Natl. Acad. Sci., 90, 8469-8473 (1973).
Fukushige et al., Proc. Natl. Acad. Sci., 89, 7905-7909 (1992).
Gage et al., J. Virol., 66, 5509-5515 (1992).
Gu et al., Cell, 73, 1155-1164 (1993).
Hasan et al., Gene, 150, 51-56 (1994).
Hoess et al., Nucleic Acids Research, 14, 2287-2300 (1986).
Holt et al., Gene, 133, 95-97 (1993).
InVitrogen (San Diego, CA) 1995 Catalog, 35.
Jalanko et al., Arch. Virol., 103, 157-166 (1988).
Jalanko et al., Biochemica et Biophysica Acta, 949, 206-212 (1988).
Jayaram, Trends in Biological Sciences, 19, 78-82 (1994).
Jung et al., Science, 259, 984-987 (1993).
Kilby et al., Trends in Genetics, 9, 413-421 (1993).
Kioussis et al., EMBO J., 6, 355-361 (1987).
Kirchmaier et al., J. Virol., 69, 1280-1283 (1995).
Kotin et al., Human Gene Therapy, 5, 793-801 (1994).
Lakso et al., Proc. Natl. Acad. Sci., 89, 6232-6236 (1992).
Landy, Current Opinion in Genetics and Development, 3, 699-707 (1993).
Levitskaya et al., Nature, 375, 685-688 (1995).
Lupton et al., Mol. Cell. Biol., 5, 2533-2542 (1985).
Middleton et al., Advances in Virus Research, 40, 19-55 (1991).
Morris et al., Nucleic Acids Res., 19, 5895-5900 (1991).
Muzyczka, Current Topics in Microbiol. and Immunol., 158, 97-129 (1992).
O'Gorman et al., Science, 251, 1351-1355 (1991).
Odell et al., Plant Physiol., 106, 447-458 (1994).
Orban et al., Proc. Natl. Acad. Sci., 89, 6861-6865 (1992).
Peakman et al., Nucleic Acids Res., 20, 495-500 (1992).
Reisman et al., Molec. Cell. Biol., 5, 1822-1832 (1985).
Rossant et al., Nature Medicine, 1, 592-594 (1995).
Sauer, Methods in Enzymol., 225, 890-900 (1993).
Sauer et al., Gene, 70, 331-341 (1988).
Sauer et al., J. Mol. Biol., 223, 911-928 (1992).
Sauer et al., Nucleic Acids Res., 17, 147-161 (1989).
Sauer et al., Proc. Natl. Acad. Sci., 84, 9108-9112 (1987).
Sauer et al., Proc. Natl. Acad. Sci., 85, 5166-5170 (1988).
Shelling et al., Gene Therapy, 165-169 (1994).
Sugden et al., J. Virol., 63, 2644-2649 (1989).
Yates et al., Nature, 313, 812-815 (1985).
Yates et al., Proc. Natl. Acad Sci., 81, 3806-3810 (1984).
Young et al., Gene, 62, 171-185 (1988).
Thrasher et al., Gene Therapy, 2, 481-485 (Sep. 1995).
Kanegae et al., Nucleic Acids Research, 23, 3816-3821 (1995).
Metzger et al., Proc. Natl. Acad. Sci., 92, 6991-6995 (1995).
Sauer et al., Methods: A Companion to Methods in Enzymology, 4, 143-149 (1992).
Watson et al. Molecular Biology of the Gene, Fourth Edition Benjamin/Cimmings Publishing Co., Inc. 639-640, 1987.
Kotin Prospects for the use of Adeno-associated virus as a vector for human gene therapy. Human Gene Therapy vol. 5 793-801, 1994.
Young et al. Utilization of an Epstein-Barr virus replicon as a eukaryotic expression vector Gene vol. 62 171-185, 1988.
Kilby et al. Site-specific recominases: tools for genome engineering Trends in Genetics vol. 9 413-421, 1993.

Affiliated with

Also associated with

Rating

Methods and vectors for site-specific recombination does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and vectors for site-specific recombination, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and vectors for site-specific recombination will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-269385