Method for fixing a protein on a pyrrole-based polymer and...

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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C435S007100, C436S524000, C436S528000, C436S532000

Reexamination Certificate

active

07998726

ABSTRACT:
The aim of the present invention is to provide a method for attaching a protein to a conductive polymer, that can be used in particular for producing a sensor or a multisensor, for example a biochip.This aim, and also others, are achieved, in accordance with the invention, by means of a method for attaching a protein to a pyrrole polymer, comprising the following steps:coupling of the protein to be attached with pyrrole so as to obtain a first solution of a protein-pyrrole coupling compound,preparation of a second solution of pyrrole not containing the protein,mixing of said first solution with said second solution so as to obtain an electropolymerization solution,electropolymerization of the pyrrole and of the protein coupled to the pyrrole on a conductive support using said electropolymerization solution.

REFERENCES:
patent: 6160103 (2000-12-01), Marchand et al.
patent: 6803228 (2004-10-01), Caillat et al.
patent: 2003/0207400 (2003-11-01), Bianchi et al.
patent: 2006/0013850 (2006-01-01), Domb
patent: A-2 750 136 (1997-12-01), None
patent: 00/36145 (2000-06-01), None
Livache et al. “Electroconducting polymers for the construction of DNA or peptide arrays on silicon chips”, Biosensors & Bioelectronics, vol. 13, pp. 629-634, XP002249768 1998.
Bidan et al. “Conducting Polymers as a link between biomolecules and microelectronics”, Synthetic Metals, vol. 102, pp. 1363-1365, XP002114817 1999.
Livache et al. “Polypyrrole DNA Chip on a Silicon Device: Example of Hepatitis C Virus Genotyping”, Analytical Biochemistry, vol. 255, pp. 188-194, XP02114813 1998.
Wolowacz et al. “Covalent Electropolymerization of Glucose Oxidase in Polypyrrole”, Anal. Chem., vol. 64, pp. 1541-1545, XP002030300 1992.
Yon-Hin et al. “Covalent Electropolymerization of Glucose Oxidase in Polypyrrole. Evaluation of Methods of Pyrrole Attachment to Glucose Oxidase on the Performance of Electropolymerized Glucose Sensors”, Anal. Chem., vol. 65, pp. 2067-2071, XP000885233 1993.
Foulds et al. “Enzyme Entrapment in Electrically Conducting Polymers”, J. Chem. Soc., Faraday Trans. 1. vol. 82, pp. 1259-1264 1986.
Guedon et al. “Characterization and Optimization of a Real-Time, Parallel, Label-Free, Polypyrrole-Based DNA Sensor by Surface Plasmon Resonance Imaging”, Anal. Chem., vol. 72, pp. 6003-6009 2000.
Carlsson et al. “Protein Thiolation and Reversible Protein-Protein Conjugation”, Biochem. J., vol. 173, pp. 723-737 1978.
Sadik et al. “Monitoring the specific adsorption of proteins using the electrochemical quartz crystal microbalance electrodes”, Talanta, vol. 55, pp. 929-941 2001.
Schuhmann. “Conducting Polymer Based Amperometric Enzyme Electrodes”, Mikrochim. Acta., vol. 121, pp. 1-29 1995.
MacBeath et al. “Printing Proteins as Microarrays for High-Throughput Function Determination”, Science, vol. 289, pp. 1760-1763 2000.

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