Purified lactase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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Details

C435S183000, C435S195000, C435S200000, C435S201000, C426S570000, C426S042000, C426S034000

Reexamination Certificate

active

07955831

ABSTRACT:
The present invention relates to a lactase solution comprising a lactase solution comprising less than 10 g/kg of poly and oligosaccharides, a process for the production of such a lactase solution from an untreated lactase solution, a sterilized lactase solution and to a process for the production of milk containing sterilized lactase, whereby such lactose is filter sterilized in-line with the milk production process.

REFERENCES:
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patent: 5962326 (1999-10-01), Shimada et al.
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patent: 1 477 087 (1977-06-01), None
patent: WO 01/04276 (2001-01-01), None
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Product Information Sheet for “Maxilact (R)” by DSM Food Specialties [online], (no date) [Retrieved Feb. 21, 2007]. Retrieved from the Internet:< URL: http://www.dsm.com/en—US/html/dfs/dairy-products-enzymes-maxilact.htm>.
NC-IUBMB (Nomenclature Committee of the International Union of Biochemistry and Molecular Biology). “Classification and Nomenclature of Enzymes by the Reactions they Catalyze.” Web version of Enzyme Nomenclature 1992 [Retrieved on Jan. 23, 2007]. Retrieved from the Internet: <URL: http:www.chem.qmul.ac.uk/iubmb/enzyme/ruls/html> (15 pages total).
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Hussein L. et al.,Journal of Food Protection, “Reduction of Lactose in Milk by Purified Lactase Produced byKluyveromyces lactis” 52(1):30-34 (1989).
Park et al.,Journal of Food Science, “Production and Characterization of Beta Galactosidase EC 3.2.1.23 fromAspergillus orizae” 44(1):100-103 (1979).
FCC, 4thed. pp. 801-802 “Lactase (neutral)(β-galactosidase) Activity” (1996).
IUBMB enzyme nomenclature EC 3.2.1.23 for β-galactosidase (1980).
IUBMB enzyme nomenclature EC 3.2.1.108 for lactase (1984).
DSM Food Specialties specification sheet for MAXILACT® LX5000 (May 2008).
Communication pursuant to Rule 114(2) EPC dated Dec. 2, 2010 with enclosed observations by a third party issued in connection with EP Appln. No. 02759791.3.
Becerra et al, “Micro-scale purification of β-galactosidase fromKluyveromyces lactisreveals that dimeric and tetrameric forms are active”, Biotechnology Techniques 12(3):253-256 (1998).
Biochemikalien Und Reagenzien Fur Die Life Science—Forschung, Sigma (1998).

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