Countercurrent tangential chromatography methods, systems,...

Details Countercurrent tangential chromatography methods, systems,... Countercurrent tangential chromatography methods, systems,...

2011-08-02

2011-08-02

Therkorn, Ernest G (Department: 1778)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S650000, C210S656000, C210S659000, C210S198200

Reexamination Certificate

active

07988859

ABSTRACT:
This invention relates to a breakthrough in the art of chromatography allowing 1) larger scale of operation; 2) faster processing time; 3) disposability; 4) reduction of media/resin expenses; and 5) a reduction of capital equipment investment. In this invention, the chromatography column is replaced by a module that consists of two or more interconnected tangential flow filters and static mixers. The chromatography resin flows through this module in a single pass, while similar operations to a regular chromatographic process are performed on the resin (binding, washing, elution, regeneration, and equilibration). The buffers for these operations are pumped into the module in a countercurrent direction to the flow of resin, and permeate solutions from later stages are recycled back into previous stages. This creates concentration gradients in the permeate solutions of the tangential flow filters in the countercurrent direction to resin flow, thus saving buffer volume and increasing process efficiency.

REFERENCES:
patent: 4780210 (1988-10-01), Hsia
patent: 6383380 (2002-05-01), Kopf
patent: 6569340 (2003-05-01), Kopf
patent: 6596172 (2003-07-01), Kopf
patent: 6946075 (2005-09-01), Kopf
patent: 2006/0118472 (2006-06-01), Schick et al.

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