Synthesis of four color 3′O-allyl, modified...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C536S024300, C536S026600, C435S006120, C435S091100, C435S091200

Reexamination Certificate

active

07982029

ABSTRACT:
This invention provides a process for making 3′-O-allyl-dGTP-PC-Biodopy-FL-510, 3′-O-allyl-dATP-PC-ROX, 3′-O-allyl-dCTP-PC-Bodipy-650 and 3′-O-allyl-dUTP-PC-R6G, and related methods.

REFERENCES:
patent: 6664079 (2003-12-01), Ju et al.
International Search Report issued by the International Searching Authority (ISA/US) on Nov. 23, 2007 in connection with International Application No. PCT/US2006/042698.
Written Opinion issued by the International Searching Authority (ISA/US) on Nov. 23, 2007 in connection with International Application No. PCT/US2006/042698.
Meng, Q. , et al. “Design and Synthesis of a Photocleavable Fluorescent Nucleotide 3′-O-Allyl-dGTP-PC-Bodipy-FL-510 as a Reversible Terminator for DNA Sequencing by Synthesis” J. Org. Chem. (2006), 71, 3248-3252.
Ruparel, H., et al. “Design and synthesis of a 3′-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis” PNAS, 2005, vol. 102, No. 17, 5932-5937.
Seo, T.S., et al. “Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides” PNAS, 2005, vol. 102, No. 17, 5926-5931.
Seo, T.S., et al. “Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry” PNAS, 2004, vol. 101, No. 15, 5488-5493.
Li, Z., et al. “A photocleavable fluorescent nucleotide for DNA sequencing and analysis” PNAS, 2003, vol. 100, No. 2, 414-419.

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