Mutated prokaryotic cells with high secretion-levels

Details Mutated prokaryotic cells with high secretion-levels Mutated prokaryotic cells with high secretion-levels

2011-05-31

2011-05-31

Rao, Manjunath N (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S252300, C435S252310

Reexamination Certificate

active

07951556

ABSTRACT:
A mutated prokaryotic cell, which secretes higher amounts of at least one heterologous polypeptide of interest and which has a reduced expression-level of YusZ or YusX, or homologues thereof, when compared with an otherwise isogenic but non-mutated cell, and methods for constructing and using such a cell in the production of polypeptides.

REFERENCES:
patent: WO 9849328 (1998-11-01), None
Bork et al. Predicting functions from protein sequence-where are the bottlenecks? Nature Genetics vol. 18 Apr. 1998.
Chen et al., Journal of Bacteriology, vol. 175, No. 17, pp. 5428-5437 (1993).
Kanamaru et al., Journal of Bacteriology, vol. 184, No. 1, pp. 43-50 (2002).

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