Method to confer formaldehyde-resistance to a plant, and a...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S288000, C800S298000, C800S306000, C800S317000

Reexamination Certificate

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07973222

ABSTRACT:
According to this invention, by introducing genes encoding hexulose-6-phosphate synthase and 6-phosphohexulose isomerase into a plant to have the genes expressed in the chloroplast of the plant, a transgenic plant having a pathway to assimilate formaldehyde through the Calvin cycle is provided. The transgenic plant according to this invention has resistance to formaldehyde and is capable of reducing the level of environmental formaldehyde significantly. Therefore, it is assumed that the transgenic plant according to this invention can be used to purify environmental condition, by placing it in a residence or in an office.

REFERENCES:
Achkor, H. et al. Plant Physiology (Aug. 2003) vol. 132; pp. 2248-2255.
Mitsui, R. et al. Journal of Bacteriology, Feb. 2000, vol. 182, No. 4; pp. 944-948.
Yurimoto, H. et al. FEMS Microbiology Letters, 2002, vol. 214; pp. 189-193.
Supplemental European Search Report, European Patent Application No. 04807024.7-1212, dated Mar. 10, 2008.
Hakima Achkor et al., “Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis”, Plant Physiology, vol. 132, No. 4, Aug. 2003, pp. 2248-2255, XP002470346.
Database EMBL [Online], Feb. 4, 2000 “Mycobacterium gastri MB19 formaldehyde-fixing operon genes (orfl, rmpB, rmpA, rmpD), partial and complete cds.” XP002470349.
Heribert Schmitz et al., “Assimilation and metabolism of formaldehyde by leaves appear unlikely to be of value for indoor air purification”, New Phytologist, vol. 147, No. 2, Aug. 2000, pp. 307-315, XP002470347.
Martina Giese et al., “Detoxification of formaldehyde by the spider plant (Chlorophytum comosum L.) cell-suspension cultures”, Plant Physiology, vol. 104, No. 4, 1994, pp. 1301-1309, XP002470348.
Izumi Orita et al., “Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase”, Applied Microbiology and Biotechnology, Springer-Verlag, BE, vol. 76, No. 2, May 23, 2007, pp. 439-445, XP019538684.
L. Chen et al., “Installation of formaldehyde-fixation pathway of methylotroph as a bypass of carboxylation step of the Calvin cycle in higher plants”, Photosynthesis Research, Dordrecht, NL, vol. 91, No. 2-3, Feb. 2007, pp. 234-234, XP008088718.
Chen LM, et al., Assimilation of formaldehyde in transgenic plants due to the introduction of the bacterial ribulose monophosphate pathway genes. Biosci Biotechnol Biochem. Mar. 23, 2010, 74(3):627-35. Epub Mar. 7, 2010, PubMed PMID: 20208346.
Song Z et al., Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde. Biotechnol Lett. Jun. 15, 2010, [Epub ahead of print] PubMed PMID:20549541.

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