Glucose dehydrogenase polypeptides and related polynucleotides

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S004000, C435S006120, C435S069100, C435S071100, C435S026000, C435S252300, C435S320100, C435S440000, C536S023200

Reexamination Certificate

active

07939309

ABSTRACT:
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.

REFERENCES:
patent: 4397952 (1983-08-01), Hoerschelmann et al.
patent: 4542098 (1985-09-01), Vandecasteele et al.
patent: 4877733 (1989-10-01), Takahashi et al.
patent: 5114853 (1992-05-01), Makino et al.
patent: 5114854 (1992-05-01), Bertholdt
patent: 5126256 (1992-06-01), Ebeling et al.
patent: 5298411 (1994-03-01), Sogabe et al.
patent: 6001615 (1999-12-01), Reeve
patent: 6344569 (2002-02-01), Mitsuda et al.
patent: 6472544 (2002-10-01), Kizaki et al.
patent: 6596879 (2003-07-01), Bosch et al.
patent: 6645746 (2003-11-01), Kizaki et al.
patent: 6689591 (2004-02-01), Müller et al.
patent: 7125693 (2006-10-01), Davis et al.
patent: 7132267 (2006-11-01), Davis et al.
patent: 1213354 (2002-12-01), None
patent: WO 00/49039 (2000-08-01), None
patent: WO 2004/015132 (2004-02-01), None
patent: WO 2005/045016 (2005-05-01), None
Baik et al., 2003, “Significantly enhanced stability of glucose dehydrogenase by directed evolution,” Appl. Micro. Biotech. 61:329-355.
Baik et al., 2005, “Cooperative Effect of Two Surface Amino Acid Mutations (Q252L and E170K) in Glucose Dehydrogenase fromBacillus megateriumIWG3 on Stabilization of Its Oligomeric State,” Appl. Environ. Microbiol. 71(6):3285-3293.
Branden et al., 1991, (Introduction to Protein Structure)Garland Publishing, Inc., New York, pp. 247.
European Search Report for EP04816807.4 dated Jun. 10, 2008.
Fortnagel et al., 1986, “Sequence Homologies of Glucose-Dehydogenases ofBacillus megateriumandBacillus subtilis,” Journal of Theoretical Biology 120(4):489-497.
Heilmann et al., 1988, “Identification and isolation of glucose dehydrogenase genes ofBacillus megateriumM1286 and their expression inEscherichia coli,” Eur J Biochem. 174(3):485-90.
International Search report for PCT/US2004/026194 dated May 10, 2005.
Ivanova et al., 2003, “Genome sequence ofBacillus cereusand comparative analysis withBacillus anthracis,” Nature, 423(6935):87-91.
Lampel, et al., Apr. 1986, “Characterization of the Developmentally RegulatedBacillus subtilisGlucose Dehydrogenase Gene,”J Bacteriol., 166(1):238-243.
Manjon, et al., Aug. 2002, “Increased Activity of Glucose Dehydrogenase Co-immobilized with a Redox Mediator in a Bioreactor with Electrochemical NAD+ Regeneration,”Biotechnol. Lett, 24(15):1227-1232.
Maurer et al., 1987, “Reversible pH-Induced Dissociation of Glucose Dehydrogenase fromBacillus megateriumII. Kinetics and Mechanism,”Z. Naturforsch. 42c, 907-915.
Mitamura et al., 1990, “Structure of Isozyme Genes of Glucose Dehydrogenase fromBacillus megateriumIAM1030”,J Ferment. Bioeng. 70(6):363-369.
Pauly et al., 1975, “D-glucose dehydrogenase fromBacillus megateriumM 1286: Purification, Properties and Structure,”Hoppe-Seyler's Z. Physiol. Chem., 356(10):1613-23.
Ramaley et al., 1983 “Glycerol protection and purification ofBacillus subtilisglucose dehydrogenase,” J. Biol. Chem. 258:12558-12565.
Vasantha, et al., 1983, “Isolation of a developmental gene ofBacillus subtilisand its expression inEscherichia coli.,” Proc. Nat. Acad. Sci. U.S.A., 80(3):785-9.
Written Opinion of Singapore Application No. 200600859-3 dated Aug. 11, 2003.
Yamamoto et al., 2001, “Crystal Structure of Glucose Dehydrogenase fromBacillus megateriumIWG3 at 1.7Å Resolution,” J. Biochem. 129:303-312.
Yamane et al., 1996, “The 25-36 region of theBacillus szlbtilischromosome: determination of the sequence of a 146 kb segment and identification of 113 genes,”Microbiology142:3047-3056.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Glucose dehydrogenase polypeptides and related polynucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glucose dehydrogenase polypeptides and related polynucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glucose dehydrogenase polypeptides and related polynucleotides will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2660035

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.