Coumarin-based cyanine dyes for non-specific protein binding

Details Coumarin-based cyanine dyes for non-specific protein binding Coumarin-based cyanine dyes for non-specific protein binding

2011-01-04

2011-01-04

Hill, Jr., Robert J (Department: 1797)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

C546S066000, C546S089000, C546S099000

Reexamination Certificate

active

07863048

ABSTRACT:
Protein dyes whose molecular structure is that of a coumarin moiety coupled to a quaternary ammonium heterocycle through a vinyl or polyvinyl linkage demonstrate the ability to associate with proteins in a non-covalent, non-specific manner at low pH, where the associated form displays a significantly higher fluorescence emission than the unassociated form. This makes the dyes useful as selective labels for proteins at the low pH and eliminates the need for the removal of extraneous components from the medium in which the proteins reside prior to detection.

REFERENCES:
patent: 5223382 (1993-06-01), Ohno
patent: 5616502 (1997-04-01), Haugland et al.
patent: 5900376 (1999-05-01), Das et al.
patent: 6750346 (2004-06-01), Czerney et al.
patent: 6972339 (2005-12-01), Lukhtanov et al.
patent: 2002/0177122 (2002-11-01), Minden et al.
patent: 2003/0165942 (2003-09-01), Czerney et al.
patent: 2004/0260093 (2004-12-01), Czerney et al.
patent: 1318177 (2004-09-01), None
Abd El-All, R. M. et al.; “Synthesis, Absorption Spectra Studies and Biological Activity of Some Novel Conjugated Dyes”; 2000,Journal of the Chinese Chemical Society, vol. 47, pp. 389-395.
Ashwell, G.J. et al.; “Improved Second-Harmonic Generation from Langmuir-Blodgett Films”; 1998,Landmuir, vol. 14, pp. 1525-1527.
Lehmann, Frank et al.; “Synthesis of Amphiphilic Styrylpyridinium and Styrylquinolinium Hemicyanines and Merocyanines”; 1995,Dyes and Pigments, vol. 29, No. 1, pp. 85-94.
Wetzl, Bianca et al.; “Set of fluorochromophores in the wavelength range from 450 to 700 nm and suitable for labeling proteins and amino-modified DNA”; 2003,Journal of Chromatography, vol. 793, pp. 83-92.
International Search Report and Written Opinion from PCT/US05/44378, dated Oct. 3, 2006 (8 pages).

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