Development of low allergen soybean seeds using molecular...

Details Development of low allergen soybean seeds using molecular... Development of low allergen soybean seeds using molecular...

2011-05-31

2011-05-31

Kapushoc, Stephen (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S023100

Reexamination Certificate

active

07951537

ABSTRACT:
A mutation in the gene encoding the P34 protein in soybean which affects allergenicity is characterized. Soybean homozygous for a mutant allele comprising a four base pair insertion at the start codon of the gene encoding the P34 protein, exhibit significantly reduced P34 protein accumulation. Nucleic acid samples of soybean may be assayed for the presence of this insertion to detect the mutant allele, and soybean containing the allele may be selected for breeding to generate reduced P34 soybean lines. Molecular markers have been developed for detecting the presence or absence of the four base pair insertion.

REFERENCES:
patent: 6864362 (2005-03-01), Kinney
GenBank Locus AB013289 (Apr. 29, 1998) ‘Glycine max gene for Bd 30K, complete cds.’ GI:3097320, from www.ncbi.nlm.nih.gov, printed pp. 1-3.
Joseph, Leina M., et al., Evaluation of Glycine Germplasm for Nulls of the immunodominant Allergent P34/Gly m Bd 30k, Crop Science, pp. 1755-1763, 46, 2006.

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