Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2000-01-07
2001-10-23
Patterson, Jr., Charles L. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S252300, C435S254100, C435S255100, C536S023200
Reexamination Certificate
active
06306635
ABSTRACT:
TECHNICAL FIELD OF THE INVENTION
The present invention relates to novel enzymes. More specifically, the invention relates to novel fungal cellulases and methods of obtaining the DNA encoding them.
BACKGROUND
There is an increasing interest in glucanases in the food and feed industries. These enzymes find application for instance in the fruit juice industry for liquefaction of plant cell wall material (pending application EP 94202442.3). They may also serve as processing aids to reduce fouling of membranes. In the feed industry the role of &bgr;-glucanases in reducing the viscosity of various sorts of grains is well established. Many of the enzyme preparations used in the food and feed area are derived from Aspergillus species, usually
Aspergillus niger
. This is a safe host which produces a large variety of enzymes such as pectinases and hemicellulases with characteristics which make them suitable for applications at moderate temperatures and at neutral to acidic pH. In contrast to pectinases and hemicellulases, cellulases are usually derived from Trichoderma. Trichoderma species such as
reesei, viride
or
longibrachiatum
are good producers of cellulolytic enzymes. However, Trichoderma enzymes cannot be used everywhere due to regulatory constraints. Thus, it would be of considerable economic value to have a good source of Aspergillus enzyrries. However, up till now, it has not been possible to clone the genes encoding glucanases from
A. niger
using the traditional method involving enzyme purification, partial amino acid sequencing and isolation of the gene or cDNA for the enzyme of interest by the derived DNA sequence.
SUMMARY
The present invention provides polydeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides which are obtainable from
Aspergillus niger var. niger
or
Aspergillus niger var. tubigensis.
REFERENCES:
patent: 5475101 (1995-12-01), Ward et al.
patent: 420 358 (1990-09-01), None
patent: 96/29415 (1996-09-01), None
Kelly et al. (1985) “Transformation ofAspergillus nigerby the amdS gene ofAspergillus nidulans,” EMBO J.4:475-479.
Sakamoto et al. (1995) “Cloning and sequencing of cellulase DNA fromAspergillus Kawachiiand its expression inSaccharomyces Cerevisiae,” Curr. Genet27:435-439.
Somogyi (1952) “Notes on Sugar Determination,”J. Biol Chem.195:19-23.
Tilburn et al. (1983). “Transformation by integration inAspergillus nidulans, ” Gene26:205-221.
de Graaff Leendert H.
van den Broeck Henriette C.
Van Ooijen Albert J. J.
Visser Jacob
Gist-Brocades B.V.
Morrison & Forester LLP
Patterson Jr. Charles L.
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