Process for the purification of nucleic acids

Details Process for the purification of nucleic acids Process for the purification of nucleic acids

1999-12-21

2001-05-22

Geist, Gary (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025410, C536S025420

Reexamination Certificate

active

06235892

ABSTRACT:

Pursuant to 35 USC §119, the priority of DE 199 00 681.4 filed Jan. 4, 1999 is claimed.
BACKGROUND OF THE INVENTION
Because of the increasing use of nucleic acids in animal and human medicine, such as in gene therapy, there has been a growing need for making these materials available in greater purity. The term “nucleic acids” is to be understood as a collective term, encompassing deoxyribonucleic acids (DNA), ribonucleic acids (RNA), antisense RNA and nucleic acids with modified bases or structure. Endotoxins from gram-negative bacteria such as
E. coli
are often present in nucleic acids and when, during the course of recovery and isolation of such acids, particles of bacterial cell walls are not completely eliminated, there exists the possibility of the endotoxins finding their way to the final nucleic acid solutions being used in, say, a medical study. The introduction of endotoxins into patients must, of course, be avoided at all costs, since, where humans and experimental animals are concerned, endotoxins cause serious symptoms of illness, including fever and inflammation of blood vessels, activate blood-clotting and stimulate the production of antigens by the immune system.
EP 0 853 123 A1 discloses a process for the purification of nucleic acids in aqueous solutions, in which the solution containing nucleic acid and not cleared of endotoxins is flowed tangentially over ultrafiltration membranes with exclusion limits of 1 to 1000 kilodaltons. When this is carried out, because of its large size, a molecule of nucleic acid should not pass the membrane. At the same time, substances of lesser molecular weight, such as endotoxins, migrate through the membrane and/or are adsorbed on the membrane. The disadvantages of such an ultrafiltration technique are the considerable cost of the necessary apparatus, the large amount of time consumed (by virtue of the relatively low filtration speed common to the use of ultrafiltration membranes), the requirement for using large volumes of liquid feed solution (making the procedure unsuitable for laboratory scale work) and the tendency for dissolved proteins to prematurely blind the membranes.
These disadvantages are overcome by the present invention, which provides a process which can be carried out quickly and simply for the purification of aqueous solutions of nucleic acids, by which a lasting reduction of the endotoxin content is achieved, and which is suitable for both large- and small-scale treatments.
BRIEF SUMMARY OF THE INVENTION
The process of the present invention comprises filtering an endotoxin-containing aqueous nucleic acid feed solution through at least one layer of a microporous weakly basic anion exchange membrane, thereby binding both endotoxins and nucleic acid(s) onto the membrane. Particles greater in size than the pores of the anion exchange membrane are excluded from passage through the membrane and are restrained on the feed side of the membrane array. Subsequently, bound nucleic acid(s) are selectively eluted from the membrane by a buffered neutral salt solution having a higher ionic strength than the nucleic acid feed solution, leaving endotoxins bound to and/or adsorbed onto the membrane. The process results in the recovery of an essentially endotoxin-free nucleic acid solution. Optionally, the nucleic acid elution step may be preceded by a washing with a buffer of lesser ionic strength than that of the elution solution, in order that impurities such as proteins, which have collected in membrane's pores or are weakly bound to the membrane may be separated therefrom.
DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS
In a preferred embodiment of the invention, the aqueous nucleic acid feed solution is adjusted to a pH in the range of 5 to 9.5 and has an ionic strength of ≦0.1M, while the pH of the buffered neutral salt solution is adjusted to a value of 5 to 9.5 with an ionic strength of from about 0.5 to about 3M. In a particularly preferred embodiment, the pH of the feed solution is adjusted to about 8 and its ionic strength is about 0.01M, while the pH of the buffered neutral salt solution is about 5.5 with an ionic strength of between about 1 and 2 M, and most preferably 0.7 M when sodium chloride is the neutral salt.
For increasing the security of the filtration and the capacity for purification, more than one layer of the membrane may be employed, particularly in the form of a laminated pack as is described in U.S. Pat. No. 5,618,418 or a wound filter as taught in DE OS 197 11 083, the pertinent disclosures of which are incorporated herein by reference. The membranes can also be in the form of one or more pleated membrane cartridges, as they are usually used in sterile filtration.
Suitable weakly basic anionic exchange membranes include those containing primary, secondary or tertiary amine groups, which are affixed to the membrane either singly or in combination with one another. Such membranes are based on immobilizing weakly basic groups onto the surface of large pore size, modified membranes such as cellulosic membranes, with diethylamine for example being the weakly basic anion exchanger. Such a membrane is commercially available from Sartorius AG of Gottingen, Germany and sold as Sartobind®D.
A preferred subset of such anion exchange membranes are those which possess predominately or exclusively tertiary aliphatic amines as the ion exchange groups, wherein at least one of the alkyl groupings contain 2 to 5 carbon atoms with those containing 3 or more carbon atoms being branched or straight chain. A particularly preferred ion exchange group is a tertiary amine, having two ethyl groups. Such membranes preferably have a pore size range of from 0.01 to 30 &mgr;m, even more preferably of from 0.1 to 8 &mgr;m. The membranes in accord with the invention should not be confused with those anion exchange membranes used in electrodialysis, even though the pore size ranges may be the same as those preferred in the present invention. See EP 0 538 315 B1 and EP 0 527 992 B1. Other membranes suitable for use in the present invention are polyamides, polysulfones and polyvinylidene fluoride.
In the filtration of an aqueous nucleic acid- and endotoxin-containing feed solution through a weakly basic anion exchange membrane, both nucleic acids and endotoxins are bound to the membrane, probably by an ionic mechanism. The binding occurs not only on the outer surface of the membrane, but also on the inner surfaces of the membrane pores.
In a rather surprising aspect of the present invention, the inventors have discovered, that upon subsequent contact with a neutral salt solution of relatively higher ionic strength than the feed solution, the nucleic acid component is selectively eluted, meaning that it is reversibly bound, while at least a majority of the endotoxin component remains irreversibly bound to and /or adsorbed onto the membrane. This effect does not occur with the use of strongly basic anion exchange membranes, such as, for example, Sartobind™ Q type membranes (Sartorius AG); specifically, while the strongly basic anion exchange membranes do bind both the nucleic acid and endotoxin components, upon subsequent contact with a neutral salt solution of a higher ionic strength both components are eluted, thus permitting endotoxins to pass through the membrane along with nucleic acid(s), an undesirable result. Porous membranes having no ion exchange groups are likewise unsuitable for purification of nucleic acids. Thus, for example, a polypropylene membrane adsorbs both endotoxins and nucleic acids irreversibly, so that selective elution of one of the two substances is not possible.
The anion exchange membranes used in the present invention exhibit a high flux, so that filtration time is held to a minimum. The use of the anion exchange membranes enables the filtration of both small and large volumes, the latter containing nucleic acids in extremely dilute concentrations. This is due to the fact that the nucleic acid molecules are ionically bound by the filtration through the membrane and can subsequently be eluted f

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