Method of detecting organisms in a sample

Details Method of detecting organisms in a sample Method of detecting organisms in a sample

1997-12-05

1999-05-11

Wortman, Donna C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 5, 435 731, 435 732, 435 733, 435 735, 435 736, 435 737, 436518, C12Q1/00;1/68;1/70

Patent

active

059027223

ABSTRACT:
The presence or absence of an organism in a sample is detected by isolating the organism from the sample by a suitable affinity matrix, releasing the organism from the affinity matrix, rupturing the cells of the organism to release total nucleic acid and hydrolyzing or digesting the total nucleic acid to form mononucleotides or individual free nucleic acid basic and inorganic phosphate to form an analyte solution, and assaying the analyte solution for the at least one presence of free nucleic acid base or inorganic phosphate to thereby determine whether the organism was present in the sample.

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Package Insert, PACE-2, Neisseria gonorrhoeae, Gen-Probe Inc, 1992.
Kato et al., Appl. Envir. Micro. 59, 3774-49 (1993).
Brindle, K.M., Biochemistry, 27 6187 (1988).

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