Method for expression and isolation of biologically active molec

Details Method for expression and isolation of biologically active molec Method for expression and isolation of biologically active molec

1995-06-05

1998-10-20

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Vector, per se

4351723, 435 691, 436 241, 935 23, 935 34, C07H 2104, C12N 1509, C12N 1563, C12N 1500

Patent

active

058245430

ABSTRACT:
A recombinant nucleic acid construct is presented in which a mouse uroplakin-II (UP-II) gene promoter is coupled to a heterologous gene of interest. When the resultant construct is introduced into a transgenic mouse, the UP-II promoter directs the expression of the heterologous gene in the urothelium of the said mouse.

REFERENCES:
Garver et al., Gene Therapy, 1(1):46-50, 1994.
Meyer-Puttlitz et al., Neuro Report, 6:1674-1678, 1995.
Lin et al., PNAS USA, 92:679-683, 1995.
Frohman et al., "Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer", Proc. Nat'l Acad. Sci. USA 1988, 85, 8998-9002.
Hicks, R.M., "The Fine Structure of the Transitional Epithelium of Rat Ureter", J. Cell Biol. 1965, 26, 25-48.
Hicks, R.M., "The Mammalian Urinary Bladder: An Accommodating Organ", Biol. Rev. 1975, 50, 215-246.
Koss, L.G., "The Asymmetric Unit Membranes of the Epithelium of the Urinary Bladder of the Rat", Lab. Invest. 1969, 21, 154-168.
Lin et al., "Precursor Sequence Processing, and Urothelium-specific Expression of a Major 15-kDa Protein Subunit of Asymmetric Unit Membrane", J. Biol. Chem. 1994, 269, 1775-1784.
Mercer et al., "The Dopamine .beta.-Hydroxylase Gene Promoter Directs Expression of E. coli lacZ to Sympathetic and Other Neurons in Adult Transgenic Mice", Neuron 1991, 7, 703-716.
Peschon et al., "Spermatid-specific expression of protamine 1 in transgenic mice", Proc. Natl. Acad. Sci. USA 1987, 84, 5316-5319.
Ryan et al., "Chromosomal localization of uroplakin genes of cattle and mice", Mamm. Genome 1993, 4, 656-661.
Staehelin, L.A., "Lumenal Plamsa Membrane of the Urinary Bladder", J. Cell Biol. 1972, 53, 73-91.
Wu, X.-R. and Sun, T.-T., "Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein", J. Cell Sci. 1993, 106, 31-43.
Wu et al., "Large Scale Purification and Immunolocalization of Bovine Uroplakins I, II, and III", J. Biol. Chem. 1990, 265, 19170-19179.
Wu et al., "Mammalian Uroplakins", J. Biol. Chem. 1990, 265, 19170-19179.
Wu et al., "Uroplakins Ia and Ib, Two Major Differentiation Products of Bladder Epithelium, Belong to a Family of Four Transmembrane Domain (4TM) Proteins", J. Biol. Chem. 1994, 269, 13716-13724.
Yu, J., "Uroplakin I: A 27-kD Protein Associated with the Asymmetric Unit Membrane of Mammalian Urothelium", J. Cell Biol. 1994, 125, 171-182.

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