Method for reducing non-specific priming in DNA amplification

Details Method for reducing non-specific priming in DNA amplification Method for reducing non-specific priming in DNA amplification

1991-12-16

1994-09-20

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

053488539

ABSTRACT:
This invention relates to a homogeneous process for amplifying a target sequence in a nucleic acid sample and detecting amplification in the absence of a separation step. The invention further provides a method for nucleic acid amplification under conditions which substantially reduce the occurrence of nonspecific amplification. Products and an apparatus related to the homogeneous process are also described.

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P. Holland et al., "Detection of specific polymerase chain reaction product . . . ", Proc. Natl. Acad. Sci. USA 88:7276-7280 (1991).
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