Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1990-02-23
1992-05-12
Russel, Jeffrey E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
424 9464, 435219, 436548, 530413, 53038825, 53038826, 935104, 935108, A61K 37547, C12N 948
Patent
active
051127547
ABSTRACT:
A novel tissue plasminogen activator having the following characteristics: molecualr weight of 65,000-72,000 Daltons as measured by SDS-PAGE electrophoresis using at 7.5% agarose gel; plasminogen activator specific activity of about 10.4.times.10.sup.4 IU/mg, wherein specific activity is defined as the ratio of fibrinolytic activity of purified t-PA measured on fibrin-agarose plates to milligrams of protein; about 83.1% absorption of t-PA by a fibrin-Sepharose column when applied; binds to a Concanavalin A column when applied; the fibrinolytic activity is substantially undiminished by heating at 60.degree. C. for 60 minutes or 95.degree. C. for 5 minutes relative to unheated t-PA; unreactive with polyclonal antisera raised against urokinase; the fibrinolytic activity is substantially stable at pH 5-10; exhibits fibrinolytic activity at pH 7.5-9.0 and temperature 39.degree.-41.degree. C.; a Km value of about 1.16.times.10.sup.-3 mol/liter and a V.sub.max of about 11.7.times.10.sup.-8 mol/liter for substrate S-2288; and fibrinolytic activity is inhibited by Co.sup.2+ Zn.sup.2+, Cd.sup.2+, Hg.sup.2+, Ni.sup.2+ and Cu.sup.2+.
REFERENCES:
patent: 4568544 (1986-02-01), Hasegawa et al.
patent: 4889808 (1989-12-01), Rappaport
Higashio Kanji
Itagaki Yasuharu
Suzuki Akira
Russel Jeffrey E.
Snow Brand Milk Products Co. Ltd.
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