Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-06
1997-07-08
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 912, 435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168, C07H 2104
Patent
active
056459947
ABSTRACT:
A method of identifying species in a sample is based on pairs of consensus amino acid segments which flank variable amino acid segments of type II DNA topoisomerases. In one embodiment, a DNA primer composition termed "universal primers", is used to amplify the DNA segments coding for the variable or "signature" amino acid sequences. The amplified segments are then cloned and sequenced, and the DNA sequence is matched against a database of signature sequences from multiple species. The signature DNA sequences may be convened to the amino acid "signature" sequences for which they code, and these signature sequences matched against a database of amino acid reference sequences, thereby determining which species were present in the original sample. The universal DNA primers are useful to detect and identify multiple unknown microbial species in a sample. In another embodiment, a specific primer pair specific for a particular organism is provided, which has sequences derived from the signature regions between the flanking conserved regions. A general method for selecting a suitable protein and producing universal primers based upon it is also provided.
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Marschel Ardin H.
University of Utah Research Foundation
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