Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-09-03
1998-12-01
Weber, Jon P.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
2101982, 210692, 423308, C12P 1934, B01D 1508, B01D 1504, C01B 1516
Patent
active
058437312
ABSTRACT:
A method is disclosed for isolating plasmid DNA, having the steps of: suspending hydroxyapatite particles in a buffer solution having a pH of about 6 to about 9, about 1 to about 100 mM of a water-soluble calcium salt and about 1 to about 100 mM of tris(hydroxymethyl) aminomethane; adding a solution containing RNA and plasmid DNA, obtained upon bacteriolysis, to the hydroxyapatite particles-containing suspension, thereby adsorbing the RNA onto the hydroxyapatite particles in the suspension; and recovering the plasmid DNA from a supernatant of the suspension. The isolation method enables effective separation of the purified plasmid DNA from a solution containing RNA and plasmid DNA in a simplified process, without the use for any special separation device.
REFERENCES:
patent: Re35267 (1996-06-01), Tsuru et al.
patent: 4781904 (1988-11-01), Tagaya et al.
patent: 4830969 (1989-05-01), Holmes
patent: 4952323 (1990-08-01), Nakabayashi et al.
patent: 4956298 (1990-09-01), Diekmann
patent: 4981952 (1991-01-01), Yan
patent: 5039408 (1991-08-01), Ichitsuka et al.
patent: 5082566 (1992-01-01), Tagaya et al.
patent: 5085781 (1992-02-01), Tsuru et al.
patent: 5098842 (1992-03-01), Nakajima et al.
patent: 5208160 (1993-05-01), Kikyotani et al.
patent: 5484720 (1996-01-01), Wurm et al.
patent: 5540995 (1996-07-01), Kitano et al.
patent: 5651884 (1997-07-01), Ichitsuka et al.
Colman et al. "Rapid purification of plasmid DNa's by hydroxyapatite chromatography," J. Biochem. (1978) 91: 303-310.
Spencer, M. "Reverse salt gradient chromatography of tRNA on unsubstituted agarose," J. Chromatog. (1982) 238: 307-316.
Lindeberg et al. "High-performance adsorption chromatography of transfer ribonucleic acids and proteins on 2-micron-spherical beads of hydroxyapatite," J. Chromatograph. (1990) 499: 153-165.
Markova et al. "Influence of silver (1+) and mercury (2+) cations on hydroxyapatite chromatography," Anal. Biochem. (1978) 84(2): 633-7.
Asahi Kogaku Kogyo Kabushiki Kaisha
Hanley Susan
Weber Jon P.
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