Homogeneous chemiluminescence immunoassay using a light absorbin

Details Homogeneous chemiluminescence immunoassay using a light absorbin Homogeneous chemiluminescence immunoassay using a light absorbin

1987-05-26

1991-05-21

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 435810, 435 793, 435975, 436172, 436518, 436808, 436810, 436826, G01N 3353, G01N 2176, G01N 33543, C12Q 128

Patent

active

050174738

ABSTRACT:
A method for separation-free solid phase immunoassay of an analyte includes contacting an antianalyte attached to the surface of a solid support with the analyte, a light absorbing material and a tracer for the analyte which includes a label. The resulting mixture is incubated and chemiluminescence is generated. All of the chemiluminescence is absorbed by the light absorbing material except that associated with the bound tracer whereby the only emission detected is due to the bound tracer. Since emission from free tracer in the fluid phase of the assay medium is not detected, separation of the bound and free fractions is unnecessary. The invention includes a kit of materials useful in performing an immunoassay in accordance with the method of the invention.

REFERENCES:
patent: 4614712 (1986-09-01), Baldwin et al.
patent: 4731337 (1988-03-01), Luotola et al.

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