Process for the injection of very small amounts of samples into

Details Process for the injection of very small amounts of samples into Process for the injection of very small amounts of samples into

1983-01-21

1985-07-30

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435240, 435241, C12Q 124, C12N 500

Patent

active

045322058

ABSTRACT:
A method and a device are proposed for the injection of very small amounts of samples into cells (74) with the aid of a microcapillary (10). During the injection, a constant injection pressure of about 0.12 bar, provided by a pressurized cylinder (46) is applied to the microcapillary (10), which is reduced after the completion of the injection to a holding pressure of 0.02 bar until the next cell (64) is to be injected. A high-pressure (p.sub.1) supplied from another pressurized cylinder (44) serves for blowing out the microcapillary tip (12). A three-way valve (42) permits simple and rapid switching between the various pressures (p.sub.1, p.sub.2 and p.sub.3). Reproducible amounts of sample in the range from 10.sup.-11 ml to 10.sup.-13 ml can be injected reproducibly, simply and rapidly.

REFERENCES:
patent: 4004718 (1977-01-01), Wesley
patent: 4111677 (1978-09-01), Andrews
Mueller et al.-Trends Biochem. Sci. (Pers. Ed.) 1980, vol. 5, No. 3, pp. 60-62.
Stacey, "Microinjection of mRNA and Other Macromolecules into Living Cells", in Buch, Methods in Enzymology, vol. 79B, pp. 76-88 (1981).
Chem. Abstracts, 92:193643t (1980).
Chem. Abstracts, 94:1675b (1981).

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