Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1993-04-23
1994-10-18
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 35, 435194, 435195, 435968, 436 55, 436 57, 436 63, 436804, 424 942, 424 946, 424 177, 424 181, C12Q 134, G01N 2300
Patent
active
053567870
ABSTRACT:
A method of identifying compounds that modulate the activity of myocardial calcium-independent phospholipase A.sub.2 is disclosed. In a test assay of the method of the invention, myocardial calcium-independent phospholipase A.sub.2 40 kDa catalytic subunit, 85kDa phosphofructokinase isoform, ATP, a substrate and a test compound are combined and the myocardial calcium-independent phospholipase A.sub.2 activity is determined. The level of activity observed in the test assay is compared to the level of activity generated from a control assay which is similar to the test assay but which does not include the test compound. Essentially pure myocardial calcium-independent phospholipase A.sub.2 85kDa phosphofructokinase isoform is also disclosed.
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Mohamed Abdel A.
Washington University
Wityshyn Michael G.
LandOfFree
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