Nucleic acid markers for rice blast resistance genes and rice bl

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 243, 536 2433, 4351723, 935 2, 935 22, 935 27, 800205, C12N 1500, C12N 1505, C12N 1510

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056749937

ABSTRACT:
The present invention provides nucleic acid markers for rice blast resistance genes, which are isolated from the rice genomic DNA, are DNA sequences having a total length of up to 2 kb, with two identical sequences of at least ten bases at the both ends thereof and are located within a distance of 2.0 cM from rice blast resistance genes Pi-b, Pi-ta or Pi-ta.sup.2 . According to the present invention, it becomes possible to easily isolate rice blast resistance genes and related genes from rice cultivars containing Pi-b, Pi-ta.sup.2 or Pi-ta, thus promoting development and breeding of superior cultivars. It becomes also possible to easily carry out a resistance test of rice. This will open up the way to create new resistance genes.

REFERENCES:
Hittalmani et al. "development of a PCR based marker to identify rice blast resistance gene, Pi-2(t), in a segregating population" Theor. Appl. Genet 91:9-14 1995.
Inukai et al. "Allelism of Blast resistace genes in near Isogenic lines of rice" Phytopathology pp. 1278-1283 1994.
Wang et al. RFLP "Mapping of Genes conferring complete and partial resistance to blast in a durably resistant rice cultivar" Genetics 13 : 1421-1434 Ap. 1994.

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