Recombinant production of chymase

Details Recombinant production of chymase Recombinant production of chymase

1992-08-12

1996-10-22

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 435 697, 435 698, 435 712, 4351723, 4353201, 4352402, 4352523, 43525233, 935 10, 935 14, 935 29, 935 47, 935 48, 935 70, 935 72, 935 73, 536 232, C12N 1557, C12N 1562, C12N 1509, C12N 1510

Patent

active

055676022

ABSTRACT:
The instant invention is directed to recombinant production of functionally active chymase. "Functionally active" as used herein refers to the ability to exhibit one or more functional activities of a full-length wild-type chymase protein. In a preferred aspect, a proteolytically inactive chymase fusion protein comprising a functionally active portion of a non-chymase protein joined to the amino-terminus of the chymase protein is produced, which, upon cleavage away of the non-chymase fusion protein portion, becomes proteolytically active. A refolding procedure for increasing yields of proteolytically active recombinant chymase is provided. The invention is further directed to use of the recombinant chymase thus produced for preparing chymase-specific antibodies.

REFERENCES:
N. K. Puwri "Refolding of recombinant porcine Growth Hormone . . . " FEBS Letts. 292(1/2)187-190 (Nov. 1991).
B. D. Korant et al. "An E. coli expression system which . . . " Biomed. Biochim. Acta. 50(4-6) 643-646 (1991).
L. F. Wang et al. "Expression in E. coli of the Bacillus . . . " Derwent Biotechnology Abstracts 10(16) 105 Abst. No. 91-09529(Aug. 1991).
C. D. O'Connor et al. "Highly Repressible Expression . . . " J. Bacteriology 169(10) 4457-4462 (Oct. 1987).
J. R. Vasquez et al. "An Expression System for Trysin" J. Cell. Biochem. 39: 265-76 (1989).
J. M. Louis et al. "Autoprocessing of the HIV-1 Protease . . . " Eur. J. Biochem 199: 361-369 (1991).
S. J. Busby et al. "Expression of Recombinant Human Plasminoger . . . " J. Biol. Chem. 266(23) 15286-15292 (Aug. 1991).
Chu et al., 1992, "Molecular Cloning and Characterization of Mouse Mast Cell Chymases", Biochimica et Biophysica Acta. 1121:83-87.
Cole et al., 1992, "Isolation and Expression of a Gene Which Encodes a Wall-Associated Proteinase of Coccidioides immitis", Infection Immunity 60:416-427.
Kaiser and Hoskin, 1992, "Expression and Utilization of Chymotrypsin-like but Not Trypsin-like Serine Protease Enzymes by Nonspecific T Killer Cells Activated by Anti-CD3 Monoclonal Antibody", Cellular Immunol. 141:84-98.
Rani et al., 1992 FASEB J., "Total Synthesis and Expression of the Dog Mast Cell Chymase Gene" 6: 1346.
Caughey et al., 1991, "Structure, Chromosomal Assignment, and Deduced Amino Acid Sequence of a Human Gene for Mast Cell Chymase", J. Bio. Chem. 266:12956-12963.
Huang et al., 1991 "Cloning and Structural Analysis of MMCP-1, MMCP-4, and MMCP-5, Three Mouse Mast Cell-Specific Serine Proteases", Eur. J. Immunol. 21:1611-1621.
Kinoshita et al., 1991, "Multiple Determinants for the High Substrate Specificity of an Angiotensin II-forming Chymase from the Human Heart", J. Biol. Chem. 266:19192-19197.
MacDougall et al., 1991, "Cloning and Expression of Protease Genes from Treponema denticola in Escherichia coli", Oral Microbiol. Immunol. 6:270-274.
McNeil et al., 1991 "Molecular Cloning of the Mouse Mast Cell Protease-5 Gene: A Novel Secretory Granule Protease Expressed Early in the Differentiation of Serosal Mast Cells", J. Biol. Chem. 266:20316-20322.
Urata et al. 1991, "Cloning of the Gene and cDNA for Human Heart Chymase", J. Biol. Chem. 266:17173-17179.
Reynolds et al., 1991 "Cloning of the cDNA and Gene of Mouse Mast Cell Protease-6", J. Biol. Chem. 266:3847-3853.
Serafin et al., 1991 "Cloning of the cDNA and Gene for Mouse Mast Cell Protease 4", J. Biol. Chem. 266:1934-1941.
Serafin et al., 1990 "Identification and Molecular Cloning of a Novel Mouse Mucosal Mast Cell Serine Protease", J. Biol. Chem. 265:423-429.
Bishop, 1990, "Gene Expression Using Insect Cells and Viruses", Current Opinion In Biotechnology 1:62-67.
Caughey et al., 1990, "Dog Mast Cell Chymase: Molecular Cloning and Characterization", Biochemistry 29:5166-5171.
Nelson and Siman, 1990, "Clipsin, a Chymotrypsin-like Protease in rat Brain Which Is Irreversibly Inhibited by .alpha.-1-Antichymotrypsin", J. Biol. Chem. 265;3836-3843.
Kitts et al., 1990, "Linearization of Baculovirus DNA Enhances the Recovery of Recombinant Virus Expression Vectors", Nucleic Acids Res. 18:5667-5672.
Schecter et al., 1990, "Identification of a Cathepsin G-like Proteinase in the MC.sub.TC Type of Human Mast Cell", J. Immunol. 145:2652-2661.
Tam and Caughey, 1990, "Degradation of Airway Neuropeptides by Human Lung Tryptase", Am. J. Respir. Cell Mol. Biol. 3:27-32.
Urata et al., 1990, "Identification of a Highly Specific Chymase as the Major Angiotensin II-forming Enzyme in the Human Heart", J. Biol. Chem. 265:22348-22357.
Birch and Loh, 1989 in "Proteolytic Enzymes: A Practical Approach" Benyon and Bonds, eds., IRL Press: Oxford, pp. 211-230.
Irani et al., 1989, "Detection of MC.sub.T and MC.sub.TC Types of Human Mast Cells by Immunohistochemistry Using New Monoclonal Anti-tryptase and Anti-chymase Antibodies", J. Histochem. Cytochem. 37:1509-1515.
Nettlebeck and Fink, 1989, "A Chymotrypsin-like Protease from Porcine Lung: Identification as Mast Cell Chymase by Immunolocalization and Partial Sequence Determination", Ber. Kernforschungsanlage Juelich, 17-20.
Nadel et al., 1989, "Roles of Mast Cell Proteases in Airways", Drugs 37 (Suppl. 1):51-55.
Ollerenshaw et al., 1989, "Absence of Immunocreative Vasoactive Intestinal Polypeptide in Tissue from the Lungs of Patients With Asthma", N. Eng. J. Med. 320:1244-1248.
Sommerhoff et al., 1989, "Mast Cell Chymase: A Potent Secretagogue for Airway Gland Serous Cells", J. Immunol 142:2450-2456.
Tam et al., 1989, "Protease Inhibitors Potentiate Vasoactive Intestinal Peptide (VIP)-Induced Relaxation In Isolated Human Airway", Am Rev. Respir. Dis. 139:A200.
Carrell, 1988, "Alzheimer's Disease: Enter A Protease Inhibitor", Nature 331:478-479.
Caughey et al., 1988, "Purification and Characterization of Dog Mastocytoma Chymase: Identification of an Octapeptide Conserved in Chymotryptic Leukocyte Proteinases", Biochem. Biophys. Acta. 952:142-149.
Benfey et al., 1987, "Cloning of the Mast Cell Protease, RMCP II: Evidence for Cell-Specific Expression and a Multi-Gene Family", J. Biol. Chem. 262:5377-5384.
Nagia et al., 1987, "Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli", Methods Enzymol. 153:461-481.
Schwartz et al., 1987, "Quantitation of Histamine, Tryptase, and Chymase in Dispersed Human T and TC Mast Cells", J. Immunol 138:2611-2615.
Irani et al., 1986, "Two Types of Human Mast Cells That Have Distinct Neutral Protease Compositions", PNAS 83:4464-4468.
Smith et al., 1984 "Human Lung Tryptase: Purification and Characterization", J. Biol. Chem. 259:11046-11051.
Schechter et al., 1983, "Human Skin Chymotrytic Proteinase. Isolation and Relation to Cathespin G and G Rat Mast Cell Proteinase I", J. Biol. Chem. 258:2973-2978.
Schwartz et al., 1981, "Tryptase from Human Pulmonary Mast Cells", J. Biol. Chem. 256:11939-11943.
Watkins, Jul. 27, 1992, "Tryptase release and clinical severity of anaesthetic reactions," Agents Actions (Special Conference Issue) C203-205.
Schwartz et al., 1987, "Tryptase levels as an indicator of mast cell activation in systemic anaphylaxis and mastocytosis," New Eng. J. Med. 316:1622-1626.
Schwartz et al., 1989, "Time course of appearance and disappearance of human mast cell tryptase in the circulation after anaphylaxi," J. Clin. Invest. 83:1551-1555.
Butrus et al., 1990, "The level of tryptase in human tears: an indicator of activation of conjunctival mast cells," Ophthalmology 97:1678-1678-1683.
Enander et al., 1991 "A new radioimmunoassay for human mast cell tryptase using monoclonal antibodies," J. Immunol. Meth. 138:39-46.
Marston, F. A. O., 1986, "The purification of eukaryotic polypeptides synthesized in Escherichia coli," Biochem. J. 240:1-12.
Pollit, S. and Zalkin, H., 1983, "Role of primary structure and disulfide bond formation in .beta.-lactamase secretion," J. Bacteriol. 153:27-32.
Fahey, R. C. et al., 1977, "On the cysteine and cystine content of proteins: Differences between intracellular and extracellular proteins," J. Mol. Evol. 10:155-160.

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