Method of immobilizing an enzyme

Details Method of immobilizing an enzyme Method of immobilizing an enzyme

1974-04-09

1976-01-06

Shapiro, Lionel M.

Textiles: fiber preparation

195 68, 195DIG11, C07G 702

Patent

active

039309509

ABSTRACT:
A method of immobilizing an enzyme is presented which comprises (1) providing an active support member which is capable of reacting with an enzyme to thereby cause the enzyme to become chemically bonded thereto and (2) contacting the active support member with an enzyme-substrate complex which has been formed by mixing together an enzyme and a specific substrate while minimizing the transformation of the substrate to product, whereby the enzyme component of the enzyme-substrate complex becomes chemically bonded to the support member. Subsequently, the substrate is removed from the enzyme-substrate complex by conventional means, such as water washing, thereby leaving the active enzyme chemically bonded to the support member.

REFERENCES:
patent: 3802997 (1974-04-01), Messing

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