Pretreatment of samples for polyiodothyronine assays

Details Pretreatment of samples for polyiodothyronine assays Pretreatment of samples for polyiodothyronine assays

1976-08-20

1978-10-24

Tanenholtz, Alvin E.

Textiles: fiber preparation

23230B, 1951035A, 424 12, G01N 3300, G01N 3100, G01N 3114

Patent

active

041219759

ABSTRACT:
Composition and method are provided for the pretreatment of serum prior to a thyroxine assay. Prior to carrying out of the assay, particularly an immunoassay or competitive protein-binding assay, the serum sample is combined with cyclodextrin preferably in combination with an hydroxylated aromatic carboxylic acid in a basic medium, in amounts sufficient to minimize interference with obtaining an accurate and reliable determination of polyiodothyronine in the serum. Normally, after the reagents have been added to the serum, a small incubation period is allowed, after which time the additional reagents may be added for carrying out the assay.

REFERENCES:
patent: 3850577 (1974-11-01), Ashkar
patent: 3928553 (1975-12-01), Hollander
patent: 3955925 (1976-05-01), Proksch et al.
Griffith et al., Advan. Catal. Related Subjs. 23, 209 (1973).

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