Immunoassay element containing a pulverized water-insoluble poly

Details Immunoassay element containing a pulverized water-insoluble poly Immunoassay element containing a pulverized water-insoluble poly

1995-02-23

1996-08-20

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 18, 435 22, 435188, 436514, 436541, 436169, 436530, 422 56, 422 60, 422 681, 422101, G01N 33535, G01N 33548

Patent

active

055478484

ABSTRACT:
An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity. When the ligand is a low molecular weight antigen, competitive reactions between the ligand, enzyme-labelled antibody and conjugate of the antigen and high molecular weight compound are utilized. When the ligand is a macromolecular antigen, a reaction between the ligand and an enzyme-labelled antibody is utilized directly. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer for detecting the thus formed diffusible material. The non-diffusible substrate is composed of a pulverized insoluble polysaccharide. The reagent layer may further contain a fragmenting enzyme for further fragmenting the non-diffusible material. Also provided are processes for quantitatively analyzing both of low molecular weight and macromolecular antigens contained in any samples by the use of the immunoassay elements of the invention.

REFERENCES:
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patent: 4337310 (1982-06-01), Batz et al.
patent: 5093081 (1992-03-01), Sudo et al.
Tietz, N. W. Textbook of Clinical Chemistry, 1986, W. B. Saunders Company, pp. 729-734.
Derwent Publications Ltd., London, GB: Class D17, AN 70-48609R & JP-B-45- 018 954 (Nagase Sangyo Co. Ltd. abstract.
Derwent Publications Ltd., London, GB; AN 8608047, & JP-A-61 031 954 (Hitachi KK) 14 Feb. 1986 abstract.

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