Methods for calcium phosphate transfection

Details Methods for calcium phosphate transfection Methods for calcium phosphate transfection

1995-07-14

1997-05-27

Ketter, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

C12N 1564

Patent

active

056331568

ABSTRACT:
A method is provided for calcium phosphate transfection of a eukaryotic host cell wherein particles comprising calcium phosphate and a desired nucleic acid are grown to an optimal size and then contacted with the host cell under conditions providing a substantially slower particle growth rate, thereby increasing the host cell's exposure to optimally-sized particles.

REFERENCES:
patent: 4968615 (1990-11-01), Koszinowski et al.
patent: 5484720 (1996-01-01), Wurm et al.
Chang, P. L., "Calcium phosphate-mediated DNA transfection" Gene Therapeutics: Methods and Applications of Direct Gene Transfer, Jon A. Wolff, Boston:Birkhauser pp. 157-179 (1994).
Chen et al., "Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA" BioTechniques 6(7): 632-638 (1988).
Chen et al., "High-efficiency transformation of mammalian cells by plasmid DNA" Molecular & Cellular Biology 7(8): 2745-2752 (1987).
Chu et al., "SV40 DNA transfection of cells in suspension: analysis of the efficiency of transcription and translation of T-antigen" Gene 13:197-202 (1981).
Czernilofsky et al., "Transformation of tobacco protoplasts by a Ca phosphate mediated precipitation technique and subsequent cellular concatemerization of the input DNA" J. Cellular. Biochem. Abstract Suppl.: p. 252, abs. No. 1776 (1985).
Donahue et al., "High efficiency DNA-mediated transformation of human diploid fibroblasts" Oncogene 3:221-224 (1988).
Graham et al., "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA" Virology 52:456-467 (1973).
Ishiura et al., "Phage Particle-Mediated Gene Transfer to Cultured Mammalian Cells" Molecular & Cellular Biology 2(6):607-616 (1982).
Kjer et al., "Efficient transfection of mosquito cells is influenced by the temperature at which DNA-calcium phosphate coprecipitates are prepared" Archives of Insect Biochem. Physiol. 16:189-200.
O'Mahoney and Adams, "Optimization of Experimental Variables Influencing Reporter Gene Expression in Hepatoma Cells Following Calcium Phosphate Transfection" DNA and Cell Biology 13(12):1227-1232 (1994).
Okayama et al., "Calcium phosphate mediated gene transfer into established cell lines" Methods in Mol. Biol., E. J. Murray, Humana Press, Inc., Chapter 2, vol. 7:15-21 (1991).
Pasco et al., "Efficient DNA-mediated gene transfer into primary cultures of adult rat hepatocytes" DNA 8(7):535-541 (1989).
Steiner et al., "An automated method for calcium phosphate-mediated gene transfer" Trends in Genetics 5(5):138 (1989).
Wigler et al., "Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor" Cell 14:725-731 (1978).
Wigler et al., "DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells" Proc. Natl. Acad. Sci. 76(3):1373-1376 (1979).
Xu et al., "High-efficiency gene transfer into cardiac myocytes" Nucleic Acids Research 20(23): 6425-6426 (1992).
Yelle et al., "Efficient transfection of mammalian cells with viral DNA in optimal culture conditions" J. Virol. Methods 7(5-6): 321-326 (1983).
Di Nocera and Dawid, "Transient expression of genes introduced into cultured cells of Drosophila" Proc. Natl. Acad. Sci. 80:7095-7098 (1983).
Wilson, Steven P. et al., "Optimization of calcium phosphate transfection for bovine chromaffin cells: relationship to calcium phosphate precipitate formation" Analytical Biochemistry 226(2):212-220 (Apr. 10, 1995).

Affiliated with

Also associated with

Rating

Methods for calcium phosphate transfection does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for calcium phosphate transfection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for calcium phosphate transfection will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2328457