Methods and products for the synthesis of oligosaccharide struct

Details Methods and products for the synthesis of oligosaccharide struct Methods and products for the synthesis of oligosaccharide struct

1995-02-23

1997-01-21

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 691, 4353201, 4351723, 4352402, 536232, C12N 910, C12N 1554

Patent

active

055959008

ABSTRACT:
A method for isolating a gene, comprising:
(i) isolating a cell possessing a post-translational characteristic of interest, said post-translational characteristic being the presence of a membrane-bound oligosaccharide or polysaccharide of interest on the surface of said cell, the presence of a soluble oligosaccharide or polysaccharide of interest in an extract of said cell, or the presence of a particularly glycosyltransferase activity in an extract of said cell;
(ii) creating a genetic library of either cDNA or genomic DNA from the genetic material of said isolated cell;
(iii) transforming host cells with said genetic library; and
(iv) screening said transformed host cells for a host cell containing said post-translational characteristic, thereby obtaining a cell containing said gene, is disclosed. The method can be used to obtain genes encoding glycosyltransferases.

REFERENCES:
patent: 4205129 (1980-05-01), Podolsky et al.
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4438200 (1984-03-01), Taubman et al.
patent: 4593004 (1986-06-01), Boross et al.
patent: 4634665 (1987-01-01), Axel et al.
patent: 4745055 (1988-05-01), Schenk et al.
patent: 5324663 (1994-06-01), Lowe
Weston et al, J. Bio. Chem., vol. 267, pp. 24575-24584 (1992).
Weston et al, J. Cell Biochem. Supp., vol. 16, part D, p. 150 (1992).
Weston et al, J. Bio. Chem., vol. 267, pp. 4152-4160 (1992).
Koszdin et al, Biochem. and Biophys. Res. Comm., vol. 187, pp. 152-157 (1992).
Nishihara et al, Biochem. and Biophys. Res. Comm., vol. 190, pp. 42-46 (1993).
Ernst et al, J. Bio. Chem., vol. 264, pp. 3436-3447 (1989).
Kukowska-Latallo et al, Genes and Development, vol. 4, pp. 1288-1303 (1990).
Larsen et al, Proc. Natl. Acad. Sci. USA, vol. 86, pp. 8227-8231 (1989).
Larsen et al, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 6674-6678 (1990).
Rajan et al, J. Bio. Chem., vol. 264, pp. 11158-11167 (1989).
Lowe et al, FASEB Abstract Form (1988).
Mulligan et al, J. Exp. Med., vol. 178, pp. 623-631 (1993).
Mulligan et al, Nature, vol. 364, pp. 149-151 (1993).
Graham et al, Virology, vol. 52, pp. 456-467 (1973).
Weber et al, Mol. Cell. Biol., vol. 8, pp. 1137-1146 (1988).
Ripka et al, Biochem. & Biophys. Res. Comm., vol. 159, pp. 554-560 (1989).
Ripka et al, (preprint) "Co-transformation . . . ".
Chao et al, Science, vol. 232, pp. 518-521 (1986).
Kavathas et al, Proc. Natl. Acad. Sci., vol. 80, pp. 524-528 (1983).
Southern et al, J. Mol. Appl. Genet., vol. 1, pp. 327-341 (1982).
Kuhn et al, Cell, vol. 37, pp. 95-103 (1984).
Wigler et al, Cell, vol. 14, pp. 725-431 (1978).
Littman et al, Cell, vol. 40, pp. 237-246 (1985).
Toone et al, Tetrahedron Rep., "Enzyme-Catalyzed Synthesis . . . " (1989).
Hodgson, Bio/Techn., vol. 8, pp. 83-111 (1990) (partial).
Stinson, C&EN, Aug. 28, 1989, pp. 25-26.
C&EN, Jan. 15, 1990, pp. 7-8.
Shaper et al, Proc. Natl. Acad. Sci., vol. 83, pp. 1573-1577 (1986).
Appert et al, Biochem. Biophys. Res. Comm., vol. 139, pp. 163-168 (1986).
Narimatsu et al, Proc. Natl. Acad. Sci., vol. 83, pp. 4720-4724 (1986).
Prieels et al, J. Biol. Chem., vol. 256, pp. 10456-10463 (1981).
Eppenberger-Castori et al, Glycoconjugate J., vol. 6, pp. 101-114 (1989).
Elices et al, J. Biol. Chem., vol. 261, pp. 6054-6072 (1986).
Blanken et al, J. Biol. Chem., vol. 260, pp. 12927-12934 (1985).
Beyer et al, J. Biol. Chem., vol. 255, pp. 5364-5372 (1980).
Beyer et al, J. Biol. Chem., vol. 255, pp. 5373-5379 (1980).
Weinstein et al, J. Biol. Chem., vol. 262, pp. 17735-17743 (1987).
Weinstein et al, J. Biol. Chem., vol. 250, pp. 13835-13844 (1982).
Creeger et al, in Meth. Ezn., V. Ginsberg, Ed., vol. 83, pp. 326-331 (1982).
E. H. Holmes et al. "Enzymatic Basis for the Accumulation . . . " J. Biol. Chem. 260(12) 7619-7627 (Jun. 1985).
E. H. Holmes et al. "Biosynthesis of the Sialyl-Le.sup.X Determinant . . . " J. Biol Chem 261(8) 3737-3743 (Mar. 1986).
A. Sanesto et al. J. Biol Chem. 267(4)2745-2752 (Feb. 1992).
R. Mollicone et al. Eur. J. Biochem. 191:169-176 (1990).

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