Nucleic acids encoding a collagenase

Details Nucleic acids encoding a collagenase Nucleic acids encoding a collagenase

1997-12-03

2000-01-25

Achutamurthy, Ponnathapu

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435440, 435226, 536 232, 536 235, C12N 120, C12N 1500, C12N 964, C07H 2104

Patent

active

060177470

ABSTRACT:
An isolated sequence is provided that encodes crab serine collagenase I. The mature recombinant collagenase (SEQ. I.D. No:4) has a molecular weight of 23,500 and may be expressed in a variety of expression systems for production of readily isolated and reliably pure collagenase with excellent specificity. The recombinant collagenase, however, may be expressed in inactive, zymogen form so as to provide long-term, shelf-stable protease that can be readily activated when collagenolytic activity is desired.

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Tsu et al., "The Substrate Specificity of Uca pugilator Collagenolytic Serine Protease 1 Correlates with the Bovine Type I Collagen cleavage Sites," J. Biol. Chem., 269:30, pp. 19565-19572 (1994).
Willett et al., "Engineered Metal Regulation of Trypsin Specificity," Biochemistry, 34 pp. 2172-2180 (1995).
Sellos et al., "Molecular Cloning of a DNA that Encodes a Serine Protease with Chymotryptic and Collagenolytic Activities in the Hepatopancreas of the Shrimp Penaeus vanameii (Crustacea, Decapoda) ,"FEBS Letters, 309:3, pp. 219-224 (Sep. 1992) .
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