Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1992-05-11
1995-10-10
Marx, Irene
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
530303, 530304, 530305, C07K 1462, C07K 14625, C12P 2106
Patent
active
054570662
ABSTRACT:
This specification describes a process for converting a human insulin precursor to human insulin, which comprises treating such human insulin precursor with trypsin and carboxypeptidase B in an aqueous medium containing per mole of human insulin precursor from about 0.1 to about 10 moles of one or more metal ions of those metals having Atomic Numbers 21 to 34, 39 to 52, 57 to 84, and 89 to 92.
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Frank Bruce H.
Heiney Richard E.
Prouty Walker E.
Walden Mark R.
Caltrider Steven P.
Eli Lilly and Company
Leeds James P.
Marx Irene
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