Immunoassay with antigen or antibody labelled microcapsules cont

Details Immunoassay with antigen or antibody labelled microcapsules cont Immunoassay with antigen or antibody labelled microcapsules cont

1986-11-07

1990-04-10

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436528, 436534, 436537, 436546, 436800, 436808, 436821, 436829, G01N 33543, G01N 33544, G01N 33546, G01N 33542

Patent

active

049160800

ABSTRACT:
Microcapsules labeled with an antigen or antibody contain a liquid containing a fluorescent substance, for example, carboxyfluorescein. The liquid in the microcapsules is prepared so as to have a viscosity different from that of a liquid outside the microcapsules. For example, the microcapsules contain polyvinyl alcohol as a substance for increasing the viscosity. Antigen-antibody reaction is caused by mixing such microcapsules, a sample and a complement, and complement is activated with resulting antigen-antibody complex, whereby the microcapsules are lysed.
The reaction mixture is irradiated with exciting light, and the polarization components (I.sub..parallel. and I.sub..perp.) of fluorescence from the reaction mixture are detected. The concentration of a substance to be assayed in the sample is determined by calculating the degree of polarization fluorescence P on the basis of the intensities of the polarization components.
According to this invention, the degree of polarization fluorescence of liquid before the immunoreaction is high but the degree of polarization fluorescence after the initiation of the immunoreaction is low. Therefore, immunoassay can be carried out with high accuracy.

REFERENCES:
patent: 4429230 (1984-01-01), Honkawa et al.
patent: 4483921 (1984-11-01), Cole
patent: 4483929 (1984-11-01), Szoka
patent: 4650770 (1987-03-01), Liu et al.
Patent Abstracts of Japan, vol. 9, No. 133 (P--362) (1856), Jun. 8, 1985; JP--A--60 17 359.
Biological Abstracts, vol. 76, No. 4, 1983, p. 2662, abstract no. 24645.

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