Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1991-08-30
1992-11-10
Weimar, Elizabeth C.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4353201, 935 25, 935 67, A01H 104, C12N 1500
Patent
active
051626012
ABSTRACT:
The present invention relates to a recombinant multigene comprising a plurality of structural genes and a plurality of DNA sequences which encode peptide linkers. In the present invention, one of the structural genes encodes a protease, the DNA sequences encoding the peptide linkers are adjacent to the DNA sequences which encode the structural genes and the peptide linkers contain an amino acid sequence which the protease recognizes as a proteolytic cleavage site. The present invention additionally relates to transgenic plants which contain such a recombinant multigene transgene, host cells transformed with such a recombinant multigene, and transgenic animals which contain such a recombinant multigene transgene. Furthermore, the present invention relates to a method of producing a plurality of polypeptides in a host by incorporating and expressing in the host a such recombinant multigene.
REFERENCES:
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Dougherty; W. G., et al., "Molecular Genetic Analysis of a Plant Virus Polyprotein Cleavage Site: A Model", 1989, Virology 171:356-364.
Dougherty, W. G., et al., "Molecular Genetic and Biochemical Evidence for the Involvement of the Heptapeptide Cleavage Sequence in Determining the Reaction Profile at Two Tobacco Etch Virus Cleavage Sites in Cell-Free Assays", 1989, Virology 172:145-155.
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Yeh, S. D., et al., "Translation of Papaya Ringspot virus RNA in Vitro: Detection of a Possible Polyprotein That is Processed for Capsid Protein, Cylindrical-Inclusion Protein and Amorphous-Inclusion Protein", 1985, Virology 143:260-271.
Crouch Deborah
DeLuca Mark
The Upjohn Company
Weimar Elizabeth C.
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