Process and reagent for the improved quantitative colorimetic de

Details Process and reagent for the improved quantitative colorimetic de Process and reagent for the improved quantitative colorimetic de

1988-01-19

1992-03-10

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, 435 28, 435190, 435191, C12Q 126

Patent

active

050949438

ABSTRACT:
The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution.
The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.

REFERENCES:
patent: 4454094 (1984-06-01), Biorling et al.
Patent Abstracts of Japan, Aug. 3, 1984, 70C236 Item #5966899.
Chem. Abstracts, vol. 102, No. 15, 15 Apr. 1985.
Yamakura (1984) Biochemical and Biophysical Research Communications, vol. 122, No. 2 (Jul. 31, 1984) pp. 635-641.

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